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ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1 – 15 of 2,091 results
1

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
GSA_VA

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2

Thermo Scientific™ Stop Solution for TMB Substrates
GSA_VA

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

EDGE
3

Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Ambient
Content And Storage Room Temperature
Form Liquid
Product Type ELISA Stop Solution
Substrate Type HRP, TMB
4

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

EDGE
Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
5

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate
GSA_VA

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

EDGE
Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
6

Thermo Scientific™ ELISA Wash Buffer (30X)

Concentrated wash buffer for efficient use of storage in the lab

EDGE
7

Invitrogen™ Wash Buffer (25X)

Suitable for use with all ELISA assays

EDGE ENCOMPASS_PREFERRED
Shipping Condition Wet Ice
8

Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.

EDGE ENCOMPASS_PREFERRED
9

R&D Systems™ Substrate Reagent Pack
GSA_VA

8 vials Color A, 8 vials Color B Product in process of being discontinued and replaced by new Next generation pack

10

Invitrogen™ IkB alpha Recombinant Rat Monoclonal Antibody (MFRDTRK), Alexa Fluor™ Plus 488

Rat Recombinant Monoclonal Antibody

EDGE ENCOMPASS_PREFERRED
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Target Species Human
Host Species Rat
Conjugate Alexa Fluor Plus 488
Applications Flow Cytometry
Form Liquid
Gene Accession No. P25963
Isotype IgG2b κ
Concentration 1 mg/mL
Antigen IkB alpha
Gene Symbols NFKBIA
Regulatory Status RUO
Purification Method Affinity chromatography
Gene Alias AI462015; ECI-6; ECI-6/IKBA; I kappa B-alpha; I(Kappa)B(alpha); I79_018146; IkappaB alpha; IkappaBalpha; I-kappaBalpha; I-kappa-B-alpha; Ikba; IKBalpha; IKB-alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappaB inhibitor alpha; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; Nfkbia; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; Rel-associated pp40; REL-associated protein pp40; RL/IF-1
Gene NFKBIA
Product Type Antibody
Gene ID (Entrez) 4792
Formulation Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
Classification Recombinant Monoclonal
Primary or Secondary Primary
Clone MFRDTRK
11

Thermo Scientific™ ELISA Blocker Blocking Buffer

Increases signal-to-noise ratio and minimizes background

12
Promotions Available

R&D Systems™ Mouse IL-1 beta/IL-1F2 DuoSet ELISA
GSA_VA

Mouse IL-1 beta/IL-1F2 DuoSet ELISA from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

Content And Storage Store the unopened product at 2°C to 8°C. Do not use past expiration date.
Conjugate Biotin
Product Type ELISA Development Kit
Gene ID (Entrez) 16176
Quantity 1 Kit
Synonym catabolin, IL-1, IL1 beta, IL-1 beta, IL1B, IL-1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Assay Range 15.60 to 1000 pg/mL
For Use With (Application) ELISA
Cross Reactivity <50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules
Species Mouse
13

Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

ENCOMPASS_PREFERRED
14

Invitrogen™ CSPD™ Substrate (0.4 mM Ready-To-Use) with Sapphire-II™ Enhancer, Size B

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

ENCOMPASS_PREFERRED
15

Invitrogen™ PON1 Recombinant Mouse Monoclonal Antibody (K56044_4E12)
GREENER_CHOICE

Mouse Recombinant Monoclonal Antibody

ENCOMPASS_PREFERRED
Content And Storage Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Target Species Human
Host Species Mouse
Conjugate Unconjugated
Applications Immunohistochemistry (Paraffin)
Form Liquid
Gene Accession No. P27169
Isotype IgG2a
Concentration 1 mg/mL
Antigen PON1
Gene Symbols PON1
Regulatory Status RUO
Purification Method Protein A
Gene Alias 2810004E20; 6330405I24Rik; A esterase 1; A-esterase 1; A-esterase 2; AI481612; AI786302; Aromatic esterase 1; aromatic esterase 2; arylesterase 1; arylesterase 2; arylesterase 3; arylesterase B-type; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; ESA; ESAesterase A; esterase A; K-45; MVCD5; paraoxanase-3; paraoxonase 1; paraoxonase 2; paraoxonase 3; paraoxonase B-type; paraoxonase nirs; PON; PON 1; PON 2; Pon1; PON2; PON3; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1; Serum aryldialkylphosphatase 2; serum paraoxonase/arylesterase 1; Serum paraoxonase/arylesterase 2; serum paraoxonase/lactonase 3
Gene PON1
Product Type Antibody
Gene ID (Entrez) 5444
Formulation PBS with 0.5% BSA, 50% glycerol and 0.09% sodium azide
Immunogen Recombinant human PON1 (aa 31-350).
Classification Recombinant Monoclonal
Primary or Secondary Primary
Clone K56044_4E12