Protein Method Blocking Buffers
RIGAKU REAGENTS Block, Wizard pH Buffer, 96 formulations, 1mL volumes
The Wizard pH Buffer screen is an optimization screen that can be applied to your crystallization hit optimization to simultaneously explore the effects of pH and buffer composition on crystal growth. The Wizard pH Buffer screen is a set of 96 unique buffer solutions encompassing 12 unique buffer systems at 8 pH points, incrementally varied by 0.4 pH units.96-well block plate formulations are 1.0 ml each in a 96 deep-well matrix block plate. All formulations are prepared with ASTM type-1 water and high-purity chemicals, 0.2 �m filtered in sterile packaging.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
502891573 PROTECTRNATM RNASE INHIBIT
Teknova 20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 8 pack.
20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 8 pack.
Teknova 10X TBS with 0.5% Tween-20. (247mM Tris, 27mM KCl, 1.37M NaCl) 1000mL, Sterile. 8 pack.
10X TBS with 0.5% Tween-20. (247mM Tris, 27mM KCl, 1.37M NaCl) 1000mL, Sterile. 8 pack.
IBA LifeSciences Biotin Blocking Buffer; 2 ml
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins such as the biotin carboxyl carrier protein (BCCP, 22 kDa) of E. coli. Biotin Blocking Buffer is recommended for blocking such biotinylated proteins on western blots prior to detection of Strep-tagII or Twin-Strep-tag fusion proteins with Strep-Tactin or Strep-TactinXT conjugates to prevent the detection of false positive bands. The buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins. Please note that usage of BioLock is not necessary when using StrepMAB-Classic, since this antibody does not bind to biotinylated proteins.