Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
Invitrogen™ iBlot™ 3 Transfer Stacks, mini, PVDF
iBlot 3 Transfer Stacks are used with iBlot 3 Western Blot Transfer Device for the transfer of proteins from an SDS-PAGE gel to a membrane support when performing western blot experiments.
Fisherbrand™ Semidry Blotting Apparatus
Sleek, low-profile system for fast, efficient Western transfers
Invitrogen™ NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels)
All buffers are made with high-purity reagents and are strictly quality controlled
Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Cytiva Whatman™ Grade GB003 Blotting Paper
Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.
Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Invitrogen™ iBlot™ 2 Transfer Stacks, PVDF, mini
iBlot 2 Transfer Stacks are used to transfer proteins using the iBlot 2 Gel Transfer Device. They are consumable stacks with an integrated pre-activated PVDF transfer membrane for dry blotting of proteins.
Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate
SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Invitrogen™ iBlot™ 3 iBind™ Flex Western Starter Kit
The iBlot 3 iBind Flex Western Starter Kit includes the iBlot 3 Western Blot Transfer Device, the iBind Flex Western Device, and all of the consumables and reagents necessary to get started using both devices for fast, efficient transfer and western processing.
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
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