Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Cytiva Whatman™ Grade GB003 Blotting Paper
Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.
Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls
Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
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Cytiva Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls
Use for robust protein blotting. Cytiva Life Sciences™ Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
Thermo Scientific™ Nitrocellulose Membranes, 0.45 μm
Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (western blotting, dot-blot assays, and other protein or nucleic acid methods).
Fisherbrand™ Semidry Blotting Apparatus
Sleek, low-profile system for fast, efficient Western transfers
Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer
Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.
Thermo Scientific™ Pierce™ Background Eliminator Kit
Rapidly remove haze and artifacts from X-ray film to correct overexposed blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography.
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
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Cytiva Amersham™ Hyperfilm™ MP
Use in automatic processors or manually in tanks and trays using most common X-ray film developers and fixers. Cytiva Life Sciences™ Amersham™ Hyperfilm™ MP is an excellent multipurpose film constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti scratch layer.
Cytiva Whatman™ Nytran™ SuperCharge Membranes
Cast uniformly on both sides of a support matrix, providing excellent symmetry and allowing membranes to lie flat without curling
Cytiva Amersham™ Hybond™ -N+ Membranes
Use for the highest sensitivity in DNA and RNA blotting. Cytiva Life Sciences™ Amersham™ Hybond™ -N+ Membrane is a positively charged nylon membranes for use with radioactive and nonradioactive chemiluminescence and chemifluorescence detection.
Cytiva Amersham™ Hybond™ PVDF Membranes: Rolls
Ideal for Western blotting applications where stripping and reprobing are necessary. Cytiva Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls are hydrophobic PVDF membranes.
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MilliporeSigma™ ReBlot Plus Kit and Reagents
Blots can be stripped of antibodies in approximately 15 minutes at room temperature