General Western Blot Reagents
Cytiva Amersham™ ECL™ start Western Blotting Detection Reagent
Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane
Promega ECL Western Blotting Substrate
The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots.
Thermo Scientific™ SuperSignal™ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-rabbit
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-mouse
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-goat
Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)
Thermo Scientific™ TMB Substrate Solution
A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)
Azure Biosystems HRP Stripping Buffer
Stripping buffer uniformly removes antibodies from HRP Western blot before reprobing with another set of antibodies.
LAMDA BIOTECH INC ECL Western Blotting Detection Kit
The ECL Western Blotting Detection Kit contains 100ml of Reagent A and 100 ml of Reagent B solution. Simply mix A and B in a 1:1 ratio right before the application. The mixture is then ready for detection of any immobilized specific antigens in chemiluminescent Western blots through horseradish peroxidase (HRP) labeled antibodies. The kit is designed for excellent sensitivity for protein level as low as femtogram. And the long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the protein signals.
LI COR BIOTECH LLC WesternSure ECL Stripping Buffer for Chemiluminescent Westerns (100 mL)
WesternSure ECL Stripping Buffer is ideal for stripping and reprobing chemiluminescent Western blots. This Western blot stripping buffer. It removes primary and secondary antibodies from PVDF or nitrocellulose membranes, maintains target antigen integrity for efficient reprobing, and it does not require hazardous shipping, unlike many other stripping buffers. Stripping buffers are not recommended if bands need to be quantified. Note: WesternSure ECL Stripping Buffer is not recommended for loading amounts over 30 µg. It will demonstrate decreased performance when used at 2X or 5X concentration and heating the solution will not increase the stripping efficiency.
Sigma Aldrich Fine Chemicals Biosciences PVDf Western Blotting Memb
PVDf Western Blotting Membranes are hydrophobic microporous polyvinylidene difluoride (PVDf) membranes with a pore size of 0.2mum. The PVDf Western Blotting Membranes are ideal for western and dot blots. The high binding capacity high mechanical strength and chemical resistance of the PVDf membrane makes it specifically useful for protein blotting. The combination of strong sample retention and low background binding generates excellent signal-to-noise ratios in colorimetric and chemiluminescent detection. The primary binding mechanism includes electrostatic and hydrophobic nature. Adsorption Goat IgG 294mug/cm2 BSA 131mug/cm2 insulin 85mug/cm2
