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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 15 of 1,449 results
1
Promotions Available

Cytiva Amersham™ ECL™ start Western Blotting Detection Reagent
GSA_VA

Reagent is luminol-based chemiluminescent substrate designed for the detection of proteins immobilized to a membrane

EDGE
Product Type ECL Western Blotting Detection Reagent
Storage Requirements 18°C to 30°C
For Use With (Application) Routine and confirmatory Western blotting
2
Promotions Available

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
GSA_VA

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

EDGE
Content And Storage Upon receipt store at room temperature. Product shipped at ambient temperature.
Form Liquid
Product Line Restore
3
Promotions Available

Thermo Scientific™ Restore™ Western Blot Stripping Buffer
GSA_VA

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

EDGE
Content And Storage Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.
Form Liquid
Product Line Restore
4

Invitrogen™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer

Use with CSPD™ or CDP-Star™ chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF

ENCOMPASS_PREFERRED
5

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

EDGE
Content And Storage Upon receipt store solutions at 4°C Products are shipped at ambient temperature.
Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence, Fluorescence, Colorimetric
6

Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.

EDGE
7

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

EDGE
Content And Storage Upon receipt store at room temperature.
Form Liquid
Product Line Restore
8

Promega ECL Western Blotting Substrate

The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots.

ENCOMPASS
Product Type ECL Western Blotting Substrate
Storage Requirements 2°C to 10°C
For Use With (Application) Detects picogram amounts of Antigen and presence of HRP, blots can be repeteadly exposed to X-ray
9

Azure Biosystems AzureSpectra Labeling Kit, for Western Blot
SDP

Kit for covalently labeling antibodies for Western blot with a fluorescent dye. Contains labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns. Each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody

Product Type Protein Labeling Reagent
Membrane Compatibility Any
Detection Method Fluorescence
10
Promotions Available

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-rabbit

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

EDGE ENCOMPASS_PREFERRED
11
Promotions Available

Invitrogen™ XCell II™ Blot Module

Easy transfer of proteins or nucleic acids from mini-gels to membranes

EDGE ENCOMPASS_PREFERRED
12
Promotions Available

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-mouse

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

EDGE ENCOMPASS_PREFERRED
13
Promotions Available

Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 kit
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Gel Compatibility Any Gel Type and Gel Chemistry
Product Line Invitrogen
14

MilliporeSigma™ ReBlot Plus Kit and Reagents
GSA_VA

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

EDGE
For Use With (Application) Western Blot
15
Promotions Available

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-goat

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

ENCOMPASS_PREFERRED