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Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.

Supplier:  Thermo Scientific™ 21050

Catalog No. PI21050

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Thermo Scientific™ Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

The Pierce Western Blot Signal Enhancer membrane treatment procedure is very simple, takes only 15 minutes and can be added to nearly any existing Western blotting protocol. The result is an increase in the intensity of target protein bands on the Western blot or detection of target proteins at levels that were previously not possible. The product is effective for signal intensification with both chemiluminescent and chromogenic substrates, especially with nitrocellulose membranes.


  • Increases protein detection – most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method

  • Improves antibody binding – the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively

  • Works for nearly any protein – signal enhancement has been demonstrated with targets such as IL-6, p53, NFkB, BRCA1 and EGF

  • Effective with any substrate – enhances both chemiluminescent (ECL) and colorimetric detection for Western blots

  • Compatible with any membrane – enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)

  • Fast, 15-minute protocol – optimized for a combination of simplicity, speed and signal enhancement for most proteins

  • Ready-to-use – no formulating or diluting necessary, and the reagents are stable for storage at room temperature


Enhancer Reagent 1, 250mL; Enhancer Reagent 2, 250mL



Western Blot Enhancer
Nitrocellulose, PVDF
500 mL
Colorimetric, Chemiluminescent
Western Blot
Upon receipt store kit at room temperature.




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For Research Use Only. Not for use in diagnostic procedures.