Gel Electrophoresis Buffers and Diluents
Bio Rad Laboratories READYPREP PROTEIN EXTRACTION K
Kit, Extraction Bio-Rad ReadyPrep Protein (Membrane I) 50 preps
AB Sciex LLC ssDNA 100-R Kit
For analysis of single-stranded DNA with linearity from 10 to 100 bases. Includes application disk, two coated capillaries, replaceable gel, and standards to perform at least 100 runs. UV detection is required to analyze unlabeled and standard oligonucleotides. Urea denaturant is included separately, enabling a naturing, replaceable gel, if desired. Qty 2: DNA capillary, 65 cm Qty 1: ssDNA 100-R Gel Qty 1: Tris-Borate Buffer Qty 1: 7 M Urea Qty 1: pd(A) 40-60 Test Mix This kit is not compatible with a PDA detector.
Vector Laboratories BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit 200 ml
BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium, forms an indigo, heat-stable reaction product that can be dehydrated, cleared, and permanently mounted. This substrate can also be used for developing blots or for in situ hybridization. With 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. The stock solutions in this kit are provided in convenient dropper bottles, in sufficient volume for 200 ml working solution.
Raybiotech Inc Lysis Buffer, 5 mL, 2X
5 mL of 2X Lysis Buffer. 2X Lysis Buffer should be diluted 2-fold with deionized or distilled water. Supplementation with protease or phosphatase inhibitors is recommended. You may use RayBiotech Protease Inhibitor Cocktail (AA-PI), RayBiotech Phosphatase Inhibitor Cocktail Set I (AA-PHI-I) or Phosphatase Inhibitor Cocktail Set II (AA-PHI-II), or the RayBiotech Phosphatase / Protease Inhibitor Set (AA-PIPHI). Alternatively, you can purchase inhibitor cocktails from another manufacturer and follow their specifications. For the first experiment, we recommend serial dilution testing, starting with a 5-fold dilution, to determine the optimal protein load for the assay. Optimal dilution depends on the abundance of target proteins and should be determined empirically.
Complement Technology Inc MgEGTA, 0.1 M MgCl2, 0.1 M EGTA, 10 ml, pH 7.3, Frozen liquid, 0.22 μm filtered, Research Use Only
MgEGTA (Magnesium EGTA) is a precisely formulated buffer solution designed for critical applications in alternative complement pathway assays. This product is a frozen liquid reagent composed of 0.1 M MgCl2 and 0.1 M EGTA, adjusted to a pH of 7.3. It is 0.22 μm filtered without preservatives, ensuring high purity and stability for sensitive research. Functions effectively to prevent complement activation via the classical and lectin pathways, making it an essential tool for studies requiring specific control over complement cascade activation.
- Composed of 0.1 M MgCl2 and 0.1 M EGTA
- Maintains a pH of 7.3
- 0.22 μm filtered for high purity
- Frozen liquid form enhances stability
- No preservatives used
- Storage temperature options: 4 °C or -20 °C or below
- Manufactured in the USA
- Intended for research use only
- Not classified as a hazardous substance
Bioworld CHAPS Lysis Buffer (2X), 50 mL
This buffer is used for cell lysis and protein extraction.1x Concentration Contains:50mM Tris-HCl (pH 7.4)110mM NaCl5mM EDTA1% CHAPS.CHAPS is 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate Warning:For laboratory use only.Not fit for agricultural, household, clinical use or humanconsumption.
Biocare Medical, LLC Da Vinci Green Diluent - 500 ml
Da Vinci Green antibody diluent is a modified PBS formulation with protein carrier and preservative. This modified PBS is formulated for superior performance and stability. In addition, it is protease and IgG-free. Da Vinci Green is Biocare's new standard-universal PBS diluent. It can also be used on an automated staining system.
Seracare Life Sciences Inc WASH SOLUTION CONCENTRATE KIT
Convenient 20X concentrate containing imidazole-buffered saline and Tween 20. Kit contains 4 x 200 ml bottles, enough to prepare 16 liters of working solution. Suitable for ELISA, blotting and immunohistochemistry applications.