Gel Electrophoresis Buffers and Diluents

Buffer and diluent solutions to prepare, dilute, and deliver protein and nucleic acid samples to electrophoresis gels for separation and analysis; includes solutions to stabilize, control the concentration of, denature, and reduce sample proteins.

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Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

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Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)

Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

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Specifications

Shipping Condition	Approved for shipment at Room Temperature or on Wet Ice
Buffer	Sample Loading Buffers
Content And Storage	Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red. Store at 4°C to 25°C.
Gel Type	NuPAGE
Product Type	LDS Sample Buffer
Gel Compatibility	NuPAGE™ Gels
Product Line	NuPAGE™

Corning™ 3D Clear Starter Kit

Corning™'s 3D Clear Starter Kit combines 30 mL of 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabeling. Only immunolabels and nuclear stains need to be added. This kit comes with enough material to process three 96-well plates of 3D cell culture models.

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Invitrogen™ Digitonin (5%)

Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.

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Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

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Specifications

Shipping Condition	Wet Ice
Content And Storage	NuPAGE™ Sample Reducing Agent (500 mM dithiothreitol) should be stored at 4°C. Guaranteed stable for 6 months unless otherwise specified in the product literature.
Product Type	Sample Reducing Agent
Shelf Life	6 Months
Concentration	500 mM
Product Line	NuPAGE™

Invitrogen™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

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Specifications

Shipping Condition	Room Temperature
Buffer	Transfer Buffers
Content And Storage	Store at room temperature. Guaranteed stable for 6 months unless otherwise specified in the product literature.
Gel Type	NuPAGE
Product Type	Transfer Buffer
Shelf Life	6 Months
Gel Compatibility	NuPAGE™ Gels
Product Line	NuPAGE™

Invitrogen™ IEF Cathode Buffer pH 3-10 (10X)

An optimized pre-mixed IEF cathod buffer

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Invitrogen™ NativePAGE™ Sample Buffer (4X)

A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System

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Invitrogen™ NativePAGE™ Sample Prep Kit

Includes sample preparation reagents for native gel electrophoresis

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Thermo Scientific™ Pierce™ Lane Marker Reducing Sample Buffer

Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker.

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Invitrogen™ Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311

Optimized for using with Novex™ pH 3-10 IEF gels

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Thermo Scientific™ Pierce™ Endotoxin-Free Magnesium Chloride (MgCl₂) (10 mM)

Pierce Endotoxin-Free MgCl₂ buffer is used to prepare samples for any LAL assay. As a diluent, it will eliminate the inhibitory chelation effects of EDTA. Contains <0.005 EU/mL endotoxin.