Gel Electrophoresis Reagents and Buffers
Boston Bioproducts Inc 0.2 M Histidine Buffer, pH 7.0, 500 ml, Amino acid buffer, 0.22-micron filtered
This 0.2 M Histidine Buffer, precisely adjusted to pH 7.0, is a high-purity, ready-to-use amino acid-based solution. Engineered for scientific applications, it effectively maintains stable pH conditions within a range of 5.0 to 8.0, making it ideal for critical procedures such as protein purification, enzyme assays, cell culture, and molecular biology techniques. The buffer is 0.22-micron filtered, ensuring reliability and reproducible results in sensitive biological research and development processes.
- Buffer concentration: 0.2 M
- Initial pH: 7.0
- Volume: 500 ml
- Buffer type: Amino acid
- Effective buffering range: pH 5.0-8.0
- Filtration: 0.22-micron
- Storage temperature: 2-8°C
- Quality control tests: Appearance, pH
- Format: Ready-to-use solution
- Primary applications: Protein purification, enzyme assays, cell culture, bioprocessing, molecular biology, drug development
Hach Company Hardness buffer solution for HACH SP510, 1l, 10 mg/l
Hardness buffer solution, 1l, 10 mg/l
Aqua Solutions Acetate Buffer pH 4.0 APHA for Chlorine (10L)
Acetate Buffer pH 4.0 APHA for Chlorine (10L)
SIMPLEX SCIENCES ss50 DNA Ladder (100µL)
The ss50 DNA Ladder is an oligo length standard, or single-stranded DNA ladder, ranging from 50 to 500 nucleotides in 50-nucleotide increments.
Hach Company SC4500 Ctrl C1D2 Prog LAN Prof
SC4500 Controller-C1D2-Prognosys-LAN Profibus DP-1 UPW pH/ORP 1 UPW Conductivity Sensor-24 VDC-without power cordIncludes Controller-Non-Claros Embedded Prognosys-without power cord includes mounting hardware
GenDEPOT Xpert Prestained Protein Marker (6.5-240 Kda), 2x250ul
Xpert Prestained Protein Marker (6.5-240 Kda), 2x250ul
Research Products International Corp Agarose HR, 100 G
For DNA/RNA fragments from 200-800bp. Provides sharp resolution and low background.
- Gel Strength, 3.0%: >500 g/cm2
- Gel Strength, 5.0%: >1,000 g/cm2
- Gelling Temperature: <31°C
- Melting Temperature: <76°C
- EEO: <0.12
- Range of Separation, 3.0%: 80-500bp
- Range of Separation, 4.0%: 30-300bp
- Range of Separation, 5.0%: 10-200bp
Bioworld Borate Buffer, 0.05M, pH 8.5, 500 mL
Borate Buffer A reagent used in electrophoresis buffer and plant tissue culture media. Technical Information:ACS GradepKa (@ 20°C): 9.24Chloride <.001%Phosphate <0.001%Sulfate <0.01%Calcium <0.005%Heavy metals (as Pb) <0.001%Iron <0.001%PH Value: 8.5Protocol: Borate Buffer pH 7.4-9.2Borax (sodium tetraborate) 0.2M = 76.2 gm/ml Na2B407*120H20 (MW = 381.37)Boric acid 0.2M = 12.37 gm/1 H3BO3 (MW = 61.83)Add boric acid to borax solution until desired pH is reached. Dilute to desired molarity with ddH20.
Biotium NucSpot 470, 1000X in DMSO
NucSpot 470 is a cell membrane-impermeant green fluorescent DNA stain. It is virtually non-fluorescent in the absence of DNA, but fluoresces bright green upon DNA binding. While other green nucleic acid stains like TOTO, TO-PRO, or SYTOX dyes stain both the nucleus and cytoplasm, NucSpot 470 specifically stains the nucleus of fixed and permeabilized cells. It also can be used to selectively stain dead cells in living cultures. NucSpot 470 has an excitation/emission of 460/546 nm and can be imaged using standard settings for FITC. Properties: Ex/Em= 460/546 nm (with DNA); Store at 4°C and protect from light.
Ubpbio M-XStableProteinLadder,6X500UL
M-XStable Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-glycine buffer). The M-XStable Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon , or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Ubpbio 100 bp DNA Ladder, 6X500UL
A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate amount of DNA in each band is provided (0.54 μg per load).
Bioworld Alkaline DNA Gel Running Buffer, 250 mL
Product DescriptionAlkaline DNA gel running buffer contains 0.5M NaOH and 50 mM EDTA. EDTA is to chelate divalent metal cations such as Mg2+ and Ca2+, which are required for DNA function of degrading enzymes (DNAses).