HiTrap(R) NHS activated HP columns are prepacked with NHS activated Sepharose(R) High Performance. This high-performance medium is designed for easy coupling of ligands containing primary amines.HiTrap(R) NHS-activated HP is a prepacked ready to use column for preparative affinity chromatography. The special design of the column together with the medium provide fast simple and easy separations in a convenient format. The column can be operated with a syringe peristaltic pump or liquid chromatography system such as AKTAdesign or fPLC(TM) System.