PCR Equipment and Supplies
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Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix, no Rox
TaqPath™ BactoPure™ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| InhibitorToleranceSimple | Very High |
|---|---|
| For Use With (Application) | Microbial Detection |
| PCR Method | qPCR |
| Multiplex Capability | Up to 4-plex |
| Detection Method | Primer-probe |
| Includes | dUTP, UNG/UDG |
| Thermostability | 72 hr., Assembled Reaction |
| Sensitivity | Down to 1 copy |
| Passive Reference Dye | None |
| For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™ 12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System |
| Sample Type | DNA |
| Reaction Speed | Fast or Standard |
| Target Specificity | cDNA or gDNA only |
| Product Line | TaqPath |
Thermo Scientific™ Phusion U Hot Start DNA Polymerases
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Product Type | Hot Start DNA Polymerase |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ dNTP Mix (2 mM each)
Thermo Scientific dNTP Mix (2 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.
| Shipping Condition | Dry Ice |
|---|---|
| Purity or Quality Grade | >99% |
| Form | Liquid |
| Label or Dye | Unlabeled |
| Concentration | 2 mM |
| For Use With (Application) | Standard PCR,High-fidelity PCR,Real Time PCR (qPCR) |
Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Content And Storage | • Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl2 • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C. |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Thermo Scientific™ Exo-Resistant Random Primer
Thermo Scientific Exonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions.
Thermo Scientific™ Phusion U Multiplex PCR Master Mixes
Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 2.5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Multiplex PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
Applied Biosystems™ Absolute Q™ 1-step RT-dPCR Master Mix (4X)
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ dITP Solution (100 mM)
Thermo Scientific dITP (2'-deoxyinosine 5'-triphosphate) is a highly stable nucleotide supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Invitrogen™ EXPRESS qPCR Supermix, universal
EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for probe-based qPCR assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Format | Tube |
| Product Type | Express qPCR Supermix |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast or Standard |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | Taq DNA Polymerase |
|---|---|
| Format | Tube |
| Product Type | qRT-PCR Kit |
| For Use With (Equipment) | 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ |
| Sample Type | RNA |
| Reaction Speed | Fast or Standard |
| PCR Method | 1-step RT-qPCR |
| Product Line | SuperScript |
| Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Gene Expression Master Mix
Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Standard |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Applied Biosystems™ VetMAX™-Plus One-Step RT-PCR Kit
For use with VetMAX™ Reagents and VetMAX™ Controls.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Gene Expression |
| PCR Method | qPCR |
| Detection Method | SYBR |
| Polymerase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
| Product Type | Real Time PCR SYBR Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Sample Type | DNA (Genomic), cDNA |
| Reaction Speed | Fast |
| Label or Dye | SYBR Green |
| Product Line | SYBR |
Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/μL)
Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus.
Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Eukaryotic 18S rRNA Endogenous Control (FAM™/MGB probe, non-primer limited)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Control Primer-Probe Set(s) |
| Gene Symbol | 18S |
| Amplicon Size | 187 |
| Concentration | 20X |
| For Use With (Application) | Gene Expression |
| PCR Method | 2-step RT-qPCR |
| Detection Method | Primer-probe |
| Accession Number | X03205.1 |
| Target | 18S rRNA |
| Format | Tube |
| Label or Dye | FAM |
| Species | Eukaryotic, Rat, Mouse, Human |
| Product Line | FAM, TaqMan |