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PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.
PCR Supplies (4,382)
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Thermal Cyclers (478)
Thermal Cycler Accessories (238)

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1 – 15 of 5,835 results
1

Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Content And Storage SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Product Type Master Mix
Form Frozen
Reaction Speed Fast or Standard
Label or Dye SYBR Green,FAM
Concentration 2X
For Use With (Application) Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method qPCR,2-step RT-qPCR,RT-qPCR
Detection Method SYBR
2

Thermo Scientific™ dNTP Mix (10 mM each)
GSA_VA

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 10 mM
3
Promotions Available

Thermo Scientific™ DreamTaq PCR Master Mixes (2X)
GSA_VA

Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.

EDGE
Shipping Condition Dry Ice
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start No
Overhang 3'-A
For Use With (Application) Standard PCR
4
Promotions Available

Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Quantity Each
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
5

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Product Type High-Fidelity DNA Polymerase
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Standard PCR,High-fidelity PCR
6

Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)

TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
InhibitorToleranceSimple High
For Use With (Application) Microbial Detection,DNA Quantitation,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability Not Applicable
Sensitivity Down to 10 copies
Format Tube
Passive Reference Dye None
For Use With (Equipment) QuantStudio™ 6 Pro or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, QuantStudio™ Absolute Q Digital PCR System, ViiA™ 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier
Sample Type DNA, RNA
Reaction Speed Fast or Standard
Target Specificity RNA only, Both RNA and DNA
Product Line TaqPath™
7

Thermo Scientific™ dNTP Set, 100 mM Solutions
GSA_VA

Thermo Scientific dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials.

EDGE
Shipping Condition Dry Ice
Purity or Quality Grade >99%
Form Liquid
Label or Dye Unlabeled
Concentration 100 mM
For Use With (Application) Standard PCR,High-fidelity PCR,Real Time PCR (qPCR)
8

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

EDGE
Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Plant
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
9

Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
GSA_VA

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.

EDGE
Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 15 to 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
10

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

EDGE
Shipping Condition Dry Ice
Polymerase Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type High-Fidelity PCR Master Mix
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2X
For Use With (Application) Standard PCR,High-fidelity PCR
11

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

EDGE
Shipping Condition Dry Ice
Polymerase Phusion Flash II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type High-Fidelity PCR Master Mix
Reaction Speed Fast
Color Colorless
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
12

Thermo Scientific™ Verso cDNA Synthesis Kit

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.

EDGE
Shipping Condition Wet or Dry Ice
Format Kit
Reverse Transcriptase Verso
Size (Final Product) Up to 11 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
13
Promotions Available

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Wet Ice
InhibitorToleranceSimple Medium
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line PowerUP™, SYBR™
14

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
InhibitorToleranceSimple High
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 8 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudio™ Absolute Q Digital PCR System, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Target Specificity cDNA or gDNA only
Product Line Applied Biosystems™
15

Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay

The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Format Kit
Label or Dye VIC
Species Not Species-Specific
Product Line VetMAX™, Xeno™