Oligonucleotides
Cayman Chemical DNA MethyltrnsfRase 1 Monc 1ea
Antigen: synthetic peptide corresponding to amino acids 637-650 of human DNMT1 • Host: mouse, clone 60B1220.1 • Isotype: IgG1κ • Cross Reactivity: (+) human, mouse, and zebrafish DNMT1 • Application(s): ChIP, IHC (paraffin-embedded sections), IP, and WB • DNMT1 is constitutively expressed in proliferating cells. DNMT1 cooperates with retinablastoma tumor suppressor to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation and cancer cell proliferation.
Cayman Chemical GW 4869 hydrochloride hydrat
An inhibitor of neutral sphingomyelinase (IC50 = 1 μM); selective for neutral sphingomyelinase over acid sphingomyelinase at concentrations up to 150 μM as well as B. cereus PC-PLC, human lyso-PAF PLC, and bovine PP2A at 10 μM; inhibits TNF-α-induced sphingomyelin hydrolysis and TNF-α-induced cell death in MCF-7 cells; reduces the inhibitory effects of OxPAPC and KOdiA-PE on LPS-induction of IL-8 in human aortic endothelial cells; reverses hypoxia-induced pulmonary vasoconstriction in rats at 1 mg/kg
CPC Scientific 5FAM-Asn-Val-Lys-Ser-Lys-Ile-Gly-Ser-Thr-Glu-Asn-Leu-Lys-NH2 (trifluoroacetate salt) 1MG
ONE-LETTER SEQUENCE: 5FAM-NVKSKIGSTENLK-NH2MOLECULAR FORMULA: C81H119N19O26MOLECULAR WEIGHT:1775STORAGE CONDITIONS: -20 5CRESEARCH AREA: Protein Kinases and Phosphatases
New England Biolabs, Inc. E. coli tRNA - 1 mg
E. coli tRNA is a mix of tRNAs isolated from E. coli and purified by chromatography to remove contaminating proteins, including nucleases, as well as other nucleic acids. This tRNA formulation sustains translation in E. coli-based in vitro protein synthesis reactions (also referred to as cell-free protein synthesis or CFPS). It is also suited for molecular biology applications that require highly pure and active prokaryotic tRNA.
- Highly active tRNA for in vitro translation
- Purified by chromatography, no organic solvents
- Highly pure with undetectable levels of nucleases and proteases
- Manufactured with reduced environmental impact
- Compatible with a variety of molecular biology workflows
CPC Scientific 5FAM-Lys-Lys-Arg-Asn-Arg-Thr-Leu-Thr-Lys-OH (trifluoroacetate salt) 0.5MG
ONE-LETTER SEQUENCE: 5FAM-KKRNRTLTKMOLECULAR FORMULA: C69H103N19O19MOLECULAR WEIGHT:1502.7STORAGE CONDITIONS: -20 5CRESEARCH AREA: Protein Kinases and Phosphatases
CPC Scientific 5FAM-Lys-Lys-Leu-Ala-Thr-Gly-Asp-Tyr-Met-Asn-Met-Ser-Pro-NH2 (trifluoroacetate salt) 0.5MG
ONE-LETTER SEQUENCE: 5FAM-KKLATGDYMNMSP-NH2MOLECULAR FORMULA: C83H113N17O25S2MOLECULAR WEIGHT:1813.1STORAGE CONDITIONS: -20 5CRESEARCH AREA: Protein Kinases and Phosphatases
New England Biolabs, Inc. NEBNext Enzymatic 5hmC-seq Kit 24 rxns
The NEBNext Enzymatic 5hmC-seq Kit specifically detects 5hmC sites. The kit includes NEBNext Ultra II library prep reagents, and 5hmC is detected using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC after Illumina sequencing. E5hmC-seq data can also be subtracted from EM-seq data, allowing determination of the precise location of individual 5mC and 5hmC sites. The kit includes the E5hmC-seq adaptor and index primers are supplied separately.
New England Biolabs, Inc. Duplex DNase - 1000 units
An engineered double-strand specific DNA endonuclease, dsDNase preferentially degrades double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA. This specificity toward dsDNA allows the enzyme to degrade contaminating dsDNA while maintaining the integrity of single stranded DNA molecules such as primers and probes within PCR mixes, allowing for efficient decontamination of PCR master mixes. Moreover, dsDNase displays the unique property of cleaving the DNA strand of a DNA:RNA hybrid duplex.
New England Biolabs, Inc. Duplex DNase - 5000 units
An engineered double-strand specific DNA endonuclease, dsDNase preferentially degrades double-stranded DNA (dsDNA) over single-stranded DNA (ssDNA) or RNA. This specificity toward dsDNA allows the enzyme to degrade contaminating dsDNA while maintaining the integrity of single stranded DNA molecules such as primers and probes within PCR mixes, allowing for efficient decontamination of PCR master mixes. Moreover, dsDNase displays the unique property of cleaving the DNA strand of a DNA:RNA hybrid duplex.
Medchemexpress LLC Odn 1826 | 202668-42-6 | 10MG
ODN 1826 is a class B CpG oligodeoxynucleotide that functions as a mouse TLR9 agonist. It is a synthetic, single-stranded 20-mer with a full phosphorothioate backbone and unmethylated CpG motifs, used to activate B cells and induce pro-inflammatory cytokines in preclinical mouse studies.
- Synthetic 20-mer oligodeoxynucleotide with a phosphorothioate backbone
- Contains unmethylated CpG motifs that activate mouse TLR9
- Potent stimulator of B cells and pro-inflammatory cytokine production
- Commonly used as a vaccine adjuvant or immunostimulant in mouse models
- Nuclease resistant for improved in vivo stability
- High purity (>98% HPLC) available and lot-tested for biological activity