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Oligonucleotides with a wide variety of applications in microarrays, gel shift assays, artificial gene synthesis, PCR, DNA sequencing, molecular cloning, and other custom procedures. Available in multiple primer lengths; may be pre-designed or random.
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The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.
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Uracil-DNA Glycosylase heat-labile contains the equally named enzyme found in the marine bacterium BMTU 3346. Like the UNG from E. coli it hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA excising uracil and creating alkali-sensitive abasic sites in the DNA. These abasic sites can be hydrolyzed by endonuclease heat or alkali treatment. Depending on how the DNA is prepared Uracil-DNA Glycosylase can be used to achieve general site-specific or strand-specific U-DNA cleavage. This enzyme shows lower thermostability and is therefore easier to inactivate.Enzyme CharacteristicsThe BMTU 3346 enzyme is inactivated more quickly (2 minutes at 95C) than the corresponding enzyme from E. coli (10 minutes at 95C). It has also been reported that UNG from E. coli remains partially active leading to the degradation of the dU-containing PCR product. In contrast the heat-labile UNG does not degrade dU-PCR products within at least several hours of incubation at 2 to 8C. Theref
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Library consists of complex biantennary oligosaccharides consistent with N-glycans on normal human IgG. The reducing termini are derivatized with the fluorescent dye, 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt (APTS) for separation by capillary electrophoresis (CE).
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Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its small size facilitates in vivo delivery for genome editing in various organisms.
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