Oligonucleotides
ABclonal Technology Rev-erb beta/NR1D2 Rabbit mAb
This gene encodes a member of the nuclear hormone receptor family specifically the NR1 subfamily of receptors The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism Alternatively spliced transcript variants have been described
New England Biolabs, Inc. Monarch® Spin Plasmid Miniprep Kit - 50 preps
The Monarch Spin Plasmid Miniprep kit, a newer version of our original Monarch Miniprep kit, offers a unique method for plasmid preparation and purification, enabling extraction of highly concentrated plasmid DNA from various bacterial strains, with yields of up to 20 ug of high-quality DNA. This kit features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 30 ul, for highly concentrated DNA in downstream application such as sequencing, restriction digestion, transformation, transfection, and more. A colored buffer system helps indicate completion of key lysis and neutralization steps for an easy to follow workflow and shorter, faster protocols allow for reduced hands-on time and less spin time. Compared to leading suppliers, these kits use significantly less plastic through uniquely designed columns and bottles with plastic reduction while still maintaining structural integrity for centrifugation or vacuum manifolds and other steps
TWD Scientific LLC Oligo dT BioXbeads 1 m Beads 1mL (5mg/mL)
Oligo dT BioXbeads 1 m Beads 1mL (5mg/mL)
TWD Scientific LLC Oligo dT BioXbeads 1 m Beads 2mL (5mg/mL)
Oligo dT BioXbeads 1 m Beads 2mL (5mg/mL)
Eagle Biosciences Inc Hela Cell Residual DNA Detection Kit (qPCR)
Hela Cell Residual DNA Detection Kit (qPCR)
New England Biolabs, Inc. Random Primer 6 – 1 A260 units
Random Primer 6 is a mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA.
New England Biolabs, Inc. Random Primer 9 – 1 A260 units
A mixture of random nonanucleotides used to prime DNA synthesis in vitro along multiple sites of denatured template DNA. The primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabeled nucleotide for the appropriate non-radioactive nucleotide in the reaction mixture. Use of synthetic d(N9) primer ensures the presence of virtually all sequence combination of nanomer primers which results in equally labelled DNA of high specific activity. Oligolabeling by this method generates probes which can be used to screen gene libraries, probe Southern and Northern blots, and for in situ hybridizations.
Enzo Life Sciences Fluorescein-12 ddUTP (25nmol)
Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies. Alternative name: Fluorescein-12-2',3'-dideoxyuridine-5'-triphosphate. Concentration: 1mM. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water. Long Term Storage: -20°C. Excitation maximum: 496 nm. Emission maximum: 520 nm.
New England Biolabs, Inc. LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG - 96 rxns
The LyoPrime Luna Probe One-Step RT-qPCR Mix with UDG is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage prior to use. The mix contains all necessary components for one-step RT-qPCR except primers, probe(s), and RNA sample. It can be reconstituted by simply adding water to a 2-4X concentration, allowing for increased sample input. Performance in multiplexing applications has been optimized, with sensitive, linear detection achieved for up to 5 targets across a range of inputs. It is available in 2 x 60 reaction glass vials or a 96-well plate (1 reaction per well).
New England Biolabs, Inc. RNase 4 - 12500 units
RNase 4 is a single-stranded RNA endonuclease that cleaves 3' of uridine in uridine-purine sequences (cut sites: U/A and U/G). RNase 4 allows more targeted digestion of substrate RNA material compared to single nucleotide specific RNases like T1 (cut site: after G), RNase U2 (cut sites: after A and G), or bovine pancreatic RNase A (cut sites: after C and U). RNase 4 endonuclease activity tolerates uridine base modifications such as pseudo-, N1-methyl-pseudo-, dihydro-, and 5-methoxy-uridine species. Due to the chemical mechanism of RNase 4 endonucleolytic cleavage, product oligonucleotides contain heterogenous 3' ends, where most species contain a linear 3'-phosphate or cyclic 2',3'-phosphate.
New England Biolabs, Inc. LyoPrime WarmStart™ Fluorescent LAMP/RT-LAMP Mix (with UDG) - 100 rxns
The LyoPrime WarmStart Fluorescent LAMP/RT-LAMP Mix (with UDG) is offered in a lyophilized format, allowing for ambient/room temperature shipping and storage prior to use. It is a simple, one-step solution for real-time detection of LAMP/RT-LAMP reactions via fluorescence. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provide rapid detection of a target nucleic acid using LAMP-specific primers and a strand-displacing DNA polymerase. Featuring a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution, this mix offers improved performance. LAMP Fluorescent Dye at 0.5X concentration is included to enable detection of amplification via real time fluorescence. Antarctic Thermolabile UDG and dUTP reduce the risk of carryover contamination between reactions. It is available in 2 x 50 reaction glass vials or a 96-well plate (1 reaction per well).