PAN-Biotech Panserin 413, Serum-free Medium for Lymphocytes, T-Cells and Hybridoma Cells, w: L-Glutamine, modified (1000 ml). MANUFACTURER: PAN-Biotech GmbH (Germany). INTRO: Panserin 413 is a ready-to-use, serum-free, low protein (<0.1%) and low animal-derived components (<0.6%) medium for serum-free cultivation and proliferation of lymphocytes from whole blood. It enables expansion of T-Cells from PBMC/whole blood after stimulation (e.g. with PHA-L or anti-CD3), expansion of NK, LAK, and CIK-cells after stimulation, and long-term cultivation of immortalized T-cells. APPLICATION: Suspension culture of T cells, CIK cells, LAK cells, or NK cells. STORAGE TEMP: +2-8°C. COMPOSITION: Defined formulation based on RPMI 1640 and DMEM/F12 is optimized with additional salts, trace elements, albumin, cholesterol, lipids and vitamins. REAGENTS NOT INCLUDED: Mitogens (ex. PHA-L), or antibodies (ex. CD3+), are necessary for cell differentiation or enrichment of T-cells from whole blood/PBMC.