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SCGM SingleQuot Kit are components sold separately and does not include the basal medium These are part of the SCGM Stromal Cell Growth Medium BulletKit (Catalog Number CC-3205) The full SCGM BulletKit consisting of basal medium and supplements can be purchased together using the catalog number CC-3205 Stromal Cell Growth Medium was developed to support the maintenance and expansion of prostate stromal cells and certain aortic and dental fibroblasts SCGM Medium contains 5% FBS Clonetics Media Products are specially designed to support the growth of human and animal primary derived cells Media systems have been specifically developed and optimized for various cell systems
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Electroporation-based methods have so far required cells to be in suspension for transfection However the 4D-Nucleofector Y Unit allows direct Nucleofection of cells in adherence Cells which typically grow in adherence in culture can be kept and transfected by Nucleofection in their physiological state The Primary Cell Optimization 4D-Nucleofector Y Kit is suited for rapid determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector Y Unit Using one 24-well Dipping Electrode Array and our pmaxGFP Vector as positive control our two Primary Cell Nucleofector Solutions for adherent Nucleofection (AD1 and AD2) are tested in combination with a pre-selected set of programs plus controls The remaining 24-well Dipping Electrode Array can be used for optional fine tuning or performing the first experiment with the selected conditions
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CDM is a chondrocyte cell differentiation basal medium sold separately This can be used with 1 kit of CC-4408 (Chondrocyte Cell Differentiation Medium SingleQuots Supplements and Growth Factors) The full CDM BulletKit consisting of basal medium and supplements can be purchased together using the catalog number CC-3225 CDM Chondrocyte Differentiation Medium is specially designed for the differentiation of chondrocytes The BulletKit is conveniently packaged with basal medium and supplements included for the differentiation of chondrocytes CGM Chondrocyte Growth Medium is also available and sold separately for expansion of human chondrocytes
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When bone marrow cells are put into culture under specific conditions a stromal layer of adherent cells developes The stromal layer is composed of many cell types including fibroblasts mesenchymal stem cells adipocytes endothelial cells and macrophages These stromal cells function as a feeder layer for hematopetic progenitors and stem cells allowing proliferation and differentiation of progenitors and stem cells to continue for weeks in these cultures with no addition of exogenous cytokines This long term culture system allows researchers to study an in vitro model of the bone marrow microenvironment including cell-cell interactions adhesion molecules and cytokine secretion These and many other factors allow for the tight regulation of blood cell production progenitor cell commitment and differentiation stem cell renewal and LTC-IC assays Available as non-irradiated only HIV-1 hepatitis B and hepatitis C are not detected for all donors and/or cell lots
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Recombinant CHO-K1 cells stably overexpress human calcitonin receptor (CT) on the surface and contain high levels of G protein G q and G s to couple with the receptor in downstream signaling pathways
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Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
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