General Cell Lines and Cell Cultures
FUJIFILM CELLULAR DYNAMICS INC CRYOPRESERVED ICELL DOPANEURON
NC3802971 CRYOPRESERVED ICELL DOPANEURON
ATCC pRS316 phagemid; in E. coli
This is one of a series of pBluescript-based YC-type (centromeric) yeast shuttle vectors (ATCC 77142-77145) differing in the yeast selectable marker gene. It encodes the beta galactosidase alpha peptide for blue-white color detection of inserts and also has the T7 and T3 promoters for in vitro RNA synthesis and priming sites for sequencing. It is useful in plasmid shuffling experiments. It was constructed by inserting a 1.112 kb fragment containing the URA3 gene into the NdeI site of the pRSS56 vector and also inserting a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) into the AatII site of the same vector. All ends were blunted. The pRSS56 vector was constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ and the fragment from the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: URA3 ? f1 ori (NaeI) ? T7 promoter ? lacZ?/MCS ? T3 promoter ? pMB1 ori ? bla ? CEN6 ? ARSH4.
ATCC Pseudomonas aeruginosa
Pseudomonas aeruginosa strain PRD-10 [CIP 103467, NCIB 10421, PCI 812] is a whole genome-sequenced strain that was isolated from a water bottle in an animal room. This bacterium can be used in disinfectant testing, antimicrobial preservative assays, testing of antimicrobial handwashing formulations, testing of slimicide, and quality control of Biosynth products. It is recommended by ATCC for use in the test described in ASTM Standard Test Method E686-91 where only the taxon is specified.
ATCC Neisseria meningitidis; serogroup y
Neisseria meningitidis strain M-112 [BO-6] is a whole-genome sequenced bacterial strain.
ATCC Odoribacter splanchnicus
Odoribacter splanchnicus strain NCTC 10825 [1651/6] is a whole-genome sequenced bacterial type strain that was isolated from an abdominal abscess.
ATCC Aspergillus sp.
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
