General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter HEK 293 GPR45 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0374C1A)
The PathHunter GPR45 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR45 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR45 and EA-tagged β-Arrestin-2. Activation of GPR45 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR27 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0334C2A)
The PathHunter GPR27 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR27 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR27 and EA-tagged β-Arrestin-2. Activation of GPR27 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Lifeline Cell Technology CELLS CORNEL EPI HU LIFELNE VL
Normal Human Corneal Epithelial Cells (HCEC), when grown in Lifeline's OcuLife Medium, provide an ideal serum-free culture model for cell-matrix interactions, gene regulation and tissue development, drug development.
Lifeline Cell Technology CELLS KERATN HU AD LIFELNE VL
Lifeline's normal Human Epidermal Keratinocytes adult are cryopreserved as primary cells. Our HEK are quality tested in DermaLife K Medium to ensure optimal serum-free growth over a period of at least 15 population doublings.
Eurofins Discoverx PathHunter U2OS EGFR/ErbB3 Dimerization Cell Line, 2 vials. (93-1125C3)
The PathHunter EGFR/ErbB3 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. heregulinβ-1) induced dimerization of EGFR/ErbB3 receptors. This product uses EFC technology. The cell line included overexpresses ErbB3 target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of EGFR/ErbB3, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter U2OS c-Ret-GFRα3 Functional Assay, 2 vials. (93-1143C3)
The PathHunter U2OS c-Ret-GFRα3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-Ret and GFRα3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged c-Ret and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, GFRα3. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the cynomolgus version of the protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter Jurkat IκB Degradation Cell Line, 2 vials. (93-1121C19)
The PathHunter Jurkat IκB Degradation Cell Line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting the NFκB pathway. This cell line enables detection of IκB levels, allowing for assessment of NFκB pathway activation-induced IκB degradation. This product uses EFC technology. The cell line included overexpresses IκB target protein fused to ProLabel. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of IκB EFC signal is lost. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Eurofins Discoverx PathHunter U2OS TGFBR1/TGFBR2/ENG Dimerization Cell Line, 2 vials. (93-1122C3)
The PathHunter TGFBR1/TGFBR2/ENG Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. TGF-β1) induced dimerization of TGFBR1/TGFBR2/ENG receptors. This product uses EFC technology. The cell line included overexpresses TGFBR1 target protein fused to PK alongside TGFBR2 target protein fused to EA and untagged ENG. Upon ligand-induced dimerization of TGFBR1/TGFBR2/ENG, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter U2OS ACVR1/ACVR2 Dimerization Cell Line, 2 vials. (93-0906C3)
The PathHunter ACVR1/ACVR2 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. BMP-7) induced dimerization of ACVR1/ACVR2 receptors. This product uses EFC technology. The cell line overexpresses truncated ACVR1 target protein fused to PK alongside truncated ACVR2 target protein fused to EA. Upon ligand-induced dimerization of ACVR1/ACVR2, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
XenoTech,LLC Pooled New Zealand rabbit liver cytosol, female, 1.0 mL at 10 mg/mL (10 mg)
Pooled New Zealand rabbit liver cytosol, female, 1.0 mL at 10 mg/mL (10 mg)
Abcam CGP 35348, GABAB antagonist, 50MG
MW 225.22 Da. Selective GABAB antagonist (IC50 = 34 μM). Brain penetrant *in vivo*. Also available in simple stock solutions (ab146707) - add 1 ml of water to get an exact, ready-to-use concentration.
The product is subject to the following: Abcam Restricted Use Statement
Abcam CGP 35348, GABAB antagonist, 10MG
MW 225.22 Da. Selective GABAB antagonist (IC50 = 34 μM). Brain penetrant *in vivo*. Also available in simple stock solutions (ab146707) - add 1 ml of water to get an exact, ready-to-use concentration.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx Ready-to-Assay CXCR3 Chemokine Receptor Frozen Cells, HEK293,2 Vials of Cells. (HTS003LRTA)
The Ready-to-Assay CXCR3 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of CXCR3, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen GIP Glucagon Receptor Membrane Preparation, 1 vial. (HTS134M)
The ChemiScreen GIP Glucagon Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of GIPR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay FSH Glycoprotein Hormone Receptor Frozen Cells, 2 Vials of Cells. (HTS178LRTA)
The Ready-to-Assay FSH Glycoprotein Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. follicle stimulating hormone) based activation of FSHR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FSHR alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FSHR, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.