General Cell Lines and Cell Cultures
Genscript Corporation CHO-K1/Cyno VISTA Stable Cell
V-domain Ig suppressor of T cell activation (VISTA) is a potent negative regulator of T-cell function that is expressed on hematopoietic cells VISTA levels are heightened within the tumor microenvironment in which its blockade can enhance antitumor immune responses in mice In humans blockade of the related programmed cell death 1 (PD-1) pathway has shown great potential in clinical immunotherapy trials
Genscript Corporation CHO-K1/SST3/G15 Stable Cell L
Recombinant CHO-K1 cells stably overexpress human somatostatin receptor 3 (SST3) on the surface and contain high levels of G protein G i to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/BDCA2 and FcER1G Stable
Recombinant CHO-K1 cells stably overexpress human C-type lectin domain family 4 member C (BDCA2) and Fc epsilon receptor Ig (FcER1G) on the surface The surface expression of BDCA2 is validated by FACS analysis
Genscript Corporation CHO-K1/NMU1 Stable Cell Line
Neuromedin U receptor 1 (NMUR1) is a member of G-protein coupled receptor family A and is the most abundant in peripheral tissue particularly in small intestines and stomach This protein is a receptor for Neuromedin U (NMU-25) The first biological activity ascribed to NmU was smooth muscle contraction Neuromedin U has also been reported to increase arterial blood pressure and modify ion transport in the intestinal tract Finally NmU injected subcutaneously into rats has been reported to result in a short term increase in circulating ACTH levels and a long term increase in serum corticosterone levels suggesting a role in regulation of the hypothalamo-pituitary-adrenal axis
Genscript Corporation CHO-K1/LTB4/G15 Stable Cell L
The dihydroxy-leukotriene leukotriene B4 (LTB4) is a member of the G protein-coupled receptor (GPCR) family in a subfamily of GPCRs that includes receptors for chemokines and other chemotactic factors Recently a second lower affinity receptor for LTB4 (BLT2) has also been cloned with broader ligand specificity for various eicosanoids LTB4 stimulates neutrophil chemotaxis and secretion but may also affect immunomodulation contraction of certain smooth muscles via an indirect mechanism and activation of the nuclear transcription factor PPAR (peroxisome proliferation activated receptor alpha) Chemotaxis the principal effects of LTB4 and related dihydroxy-acids on leukocytes occurs via activation of BLT1 receptors
Genscript Corporation CHO-K1/CD155 Stable Cell Line
CD155 commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5) is a type I transmembrane single-span glycoprotein and belongs to the nectins and nectin-like (Necl) subfamily CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV) an etiologic agent of the central nervous system disease poliomyelitis The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells CD155 may assist in an efficient humoral immune response generated within the intestinal immune system It s been shown that CD155 can be recognized and bind by DNAM-1 and CD96 which promotes the adhension migration and NK-cell killing Thus inducing cell-mediated tumor-specific immunity
Genscript Corporation CHO-K1/CD32C 13Gln Stable Cell
Recombinant CHO-K1 cells stably overexpress Homo sapiens cell-type natural killer cells Fc gamma receptor IIc1 (Fc RIIc / CD32C) on the cell surface The surface expression of Fc RIIc is validated by FACS analysis This stable cell line is recommended for evaluating the binding affinity between Fc RIIc and Fc region of antibodies Fc fusion proteins or antibody-based biologics
Genscript Corporation CHO-K1/VPAC1/G15 Stable Cell
The widespread neuropeptide vasoactive intestinal peptide (VIP) has two receptors VPAC1 and VPAC2 The vasoactive intestinal polypeptide receptor VPAC1 is G sub s /sub -coupled GPCRs expressed in the lung prostate peripheral blood leukocytes liver brain small intestine colon heart spleen placenta kidney thymus and testis
Genscript Corporation CHO-K1/MTLR/G15 Stable Cell L
The motilin receptor (MTLR) represents a clinically useful pharmacological target as agonists binding to the MTLR have gastroprokinetic properties Motilin is a 22 amino acid peptide that potently stimulates gastrointestinal contractility The biological effects of motilin are mediated by a G sub q /sub -coupled seven transmembrane protein currently termed motilin receptor (MR) that shares significant sequence similarity with the ghrelin receptor The motilin receptor is also activated by the antibiotic erythromycin this interaction appears to mediate some of the gastrointestinal side effects of erythromycin Although motilides (non-antibiotic derivatives of erythromycin) such as ABT-229 have been investigated for treatment of diabetic gastroporesis the effectiveness has been limited by tachyphylaxis (decreased response to ligand) resulting from receptor downregulation Agonists of the motilin receptor with reduced densensitization activity remain a potential treatment for dis
Genscript Corporation CHO-K1/OX40 Stable Cell Line
OX40 (CD134) is a member of the TNFR/TNF superfamily Costimulatory signals from OX40 to a conventional T cell promotes division and survival augmenting the clonal expansion of effector and memory populations as they are being generated to antigens OX40 additionally suppresses the differentiation and activity of Treg further amplifying this process
Genscript Corporation CHO-K1/ADRB1/G15 Stable Cell
The -adrenergic receptors are linked to G proteins The -receptor has three known subtypes Beta-1 receptors primarily regulate myocardial tissue and affect the rate of contraction via impulse conduction Beta-2 receptors regulate smooth muscle tone and influence vascular and bronchiolar relaxation Beta-3 receptors are less well studied but are thought to primarily affect lypolysis and may have effects on cardiac inotropy (Greene Shepherd 2006) In the human heart beta (1)- and beta (2)AR are the most powerful physiologic mechanism to acutely increase cardiac performance Changes in betaAR play an important role in chronic heart failure (CHF) Thus due to increased sympathetic activity in CHF betaAR are chronically (over) stimulated and that results in beta (1) AR desensitization and alterations of down-stream mechanisms (Brodde OE 2006)
Genscript Corporation CHO-K1/GHSR Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human growth hormone secretagogue receptor (GHSR) on the surface and contain high levels of G protein G q to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/GALR1/G15 Stable Cell
The neuropeptide galanin elicits a range of biological effects by interacting with specific G-protein-coupled receptors Galanin receptors are seven-transmembrane proteins that have been shown to activate a variety of intracellular second-messenger pathways GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family GALR1 is widely expressed in the brain spinal cord and peripheral sites including small intestine and heart
Lifeline Cell Technology CELLS LUNG SM HU LD LIFELN VL
Lifeline's normal Human Lung Smooth Muscle Cells, when grown in VascuLife SMC Medium, provide an ideal culture model for the study of angiogenesis, atherosclerosis, diabetes or vascular/pulmonary biology.
Lifeline Cell Technology CELLS HUVEC LIFELINE VL
Normal Human Umbilical Vein Endothelial Cells (HUVEC), cryopreserved as primary cells. Quality tested in VascuLife 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings.