General Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen C3aR Anaphylotoxin Receptor Membrane Preparation, 1 vial. (HTS016M)
The ChemiScreen C3aR Anaphylotoxin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of C3AR1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PathHunter U2OS HTR2C β-Arrestin Cell Line, 2 vials. (93-0289C3)
The PathHunter HTR2C cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C and EA-tagged β-Arrestin-2. Activation of HTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GPR31 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0406C2)
The PathHunter GPR31 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR31 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR31 and EA-tagged β-Arrestin-2. Activation of GPR31 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Cell Applications Inc Pre-Screened Human Lung Microvascular Endothelial Cells Total Kit
Pre-Screened Human Lung Microvascular Endothelial Cells Total Kit:(S540-05a) Pre-Screened Cryopreserved (S-HLMVEC), 1 vial, adult(111-500) Microvascular Endothelial Cells Growth Media 500 ml(121-25-02) Extracellular Matrix Protein Coated T-25 Flasks (2 ea)(123-100) Attachment Factor Solution 100ml(090K) Subculture Reagent Kit
Eurofins Discoverx cAMP Hunter CHO-K1 OPRD1 Gi Cell Line, 2 vials. (95-0108C2)
The cAMP Hunter OPRD1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. DADLE, DPDPE) based activation of OPRD1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRD1, a naturally Gi/Go coupled GPCR. Upon activation of OPRD1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC SNU-C2A; Colorectal Carcinoma; Human (Homo sapiens)
SNU-C2A is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The cell line is from an Asian, 43-year-old, female patient with colorectal cancer and was obtained from a third passage nude mouse xenograft of tumor fragments and can be used in cancer research.
ATCC Primary Uterine; Fibroblast Cells; Normal, Human
Primary uterine fibroblast cells are spindle-shaped and were isolated from the uterus of a donor, and can be used in female reproductive biology, drug testing, and oncology studies.
ATCC PANC-1-Luc2; Pancreatic Carcinoma; Human (Homo sapiens)
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
Eurofins Discoverx InCELL Hunter HEK 293 PIKFYVE Kinase Binding Cell Line, 2 vials. (96-0078C1)
The InCELL Hunter HEK 293 PIKFYVE Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of PIKFYVE via detection of PIKFYVE levels. This product uses EFC technology. The cell line included overexpresses PIKFYVE target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PrecisION hKv7.2/hKv7.3 Recombinant Stable Cell Line, 2 vials. (CYL3059)
The PrecisION hKv7.2/hKv7.3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv7.2/Kv7.3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv7.2/Kv7.3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx cAMP Hunter CHO-K1 ADRA2A Gi Cell Line, 2 vials. (95-0126C2)
The cAMP Hunter ADRA2A Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2A, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS CD200 Ligand Cell Line, 2 vials. (93-1137C3)
The PathHunter CD200 Ligand cell line is a stable, engineered cell line designed for use with CD200R Checkpoint Signaling Cell Lines. When co-cultured, the CD200 expressed in this cell line will induce activity of CD200R receptors, which may be assayed through SH2 domain protein recruitment. This product is a companion product designed to work with Immunoglobulin Superfamily Checkpoint Assays. The cell line included overexpresses CD200 cell surface ligand. When co-cultured with the CD200R Checkpoint Signaling Cell Line, the CD200 ligand binds to the checkpoint receptor, inducing receptor activity. This receptor activity leads to recruitment of the relevant SH2 domain protein to the checkpoint receptor inside the receptor cell line and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Brain: Cerebellar Peduncles, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Santa Cruz Biotechnology Asialoglycoprotein Receptor 1/2/ASGR1/ASGR2 Antibody (E-1) 200 µg/ml
Asialoglycoprotein Receptor 1/2/ASGR1/ASGR2 Antibody (E-1) is a mouse monoclonal IgG2b κ Asialoglycoprotein Receptor 1/2/ASGR1/ASGR2 antibody, cited in 3 publications, provided at 200 µg/mlraised against amino acids 1-291 representing full length ASGPR1 of human originAsialoglycoprotein Receptor 1/2/ASGR1/ASGR2 Antibody (E-1) is recommended for detection of ASGPR1, ASGPR2 and MASGP-BP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Lonza Walkersville NHEK-Neo – Human Epidermal Keratinocytes, Neonatal, Pooled
Cryopreserved Normal Human Epidermal Keratinocytes from pooled donros are guaranteed through 18 population doublings for adult and neonatal cells.All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Content:1 x Cryopreserved ampule of Human Neonatal Epidermal Keratinocytes containing ≥ 500,000 cells