General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS PRβ Nuclear Translocation Cell Line, 2 vials. (93-0484C3)
The PathHunter PRβ Nuclear Translocation cell line is a stable, engineered cell line for use in studying drug candidates affecting NHR activation and nuclear translocation. This cell line enables assessment of ligand (e.g. testosterone, norgestrel) based NHR activation via detection of resulting PRβ (progesterone receptor beta) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged PRβ NHR and nuclear localized EA. Upon activation of PRβ, induced nuclear translocation leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/nhr.
ATCC SV-HUC-1; Uroepithelium; Human (Homo sapiens)
SV-HUC-1 is an epithelial cell isolated from the uroepithelium of an 11-year-old, male. This line was established by the transformation of normal ureter tissue with the SV40 virus.
Biochain Institute Inc Frozen Tissue Section - Progressive Supranuclear Palsy: Brain: Frontal Lobe, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
ATCC Methanosarcina mazei; genomic DNA
Genomic DNA isolated from Methanosarcina mazei strain DSM 3647. This bacterial strain is also available as ATCC Catalog No. BAA-159™.
Biochain Institute Inc FDA Standard Tissue Array, 1; slides/PK
BioChain provides FDA Standard Frozen Tissue Array (TMA) which is designed in conformance with FDA guideline and meeting the requirements for IHC (Immunohistochemistry) and IVD (In vitro Diagnostic Devices) certification. This tissue array has two slides which contain 30 different human normal tissue types and 3 donors per tissue type. It also contains the 41 most popular human tumor tissue samples.
ATCC Ralstonia pickettii
Ralstonia pickettii strain [ICPB 3981, K-288] was isolated from a patient who had undergone a tracheotomy. This culture is a whole-genome sequenced bacterial type strain.
ATCC RS4 (11); Leukemia; Human (Homo sapiens)
RS4;11 is a lymphoblast cell line that was isolated from the marrow of a White, 32-year-old, female patient with acute lymphoblastic leukemia. This cell line was deposited by JH Kersey. It can be used in immunology and immune system disorder research.
Biochain Institute Inc Paraffin Tissue Section - Human Adult Normal: Heart: Arcus Aortae, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx Ready-to-Assay BB3 Bombesin Receptor Frozen Cells, 2 Vials of Cells. (HTS160RTA)
The Ready-to-Assay BB3 Bombesin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. bombesin) based activation of BRS3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses BRS3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of BRS3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx PathHunter FPRL1 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0666C3)
The PathHunter FPRL1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of FPRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged FPRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting FPRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
STEMCELL Technologies Human Peripheral Blood CD4+CD25+CD127low T cells, Frozen, Size: 2 x 10^6 cells
Primary human CD4+CD25+CD127low T cells were isolated from peripheral blood using immunomagnetic separation techniques. CD4+, CD25+, and CD127low are expressed on specific subsets of T cells.Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
ACROBiosystems HEK293/Human FOLR1 Stable Cell Line
Folate Receptor 1 (FOLR1) is also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
Cell Systems Corporation Primary Human Glomerular Mesangial Cells (ACBRI 127 V)
Primary Human Glomerular Mesangial Cells (ACBRI 127 V) were initiated by elutriation of dispase dissociated decapsulated glomeruli isolated from normal human cortical kidney tissue. Unlike cells from other providers, our cells are isolated without antibody labels. Each vial contains ~1 x 10^6 cells with 85-92% viability under ideal conditions. They are available at Passage 3 (<12 cumulative population doublings) and will initiate a Passage 4 cell culture in a 75 cm2 flask. Each vial of cells is shipped with Bac-Off (antibiotic) and CultureBoost (animal derived growth factors) at no additional cost. You can enhance consistency in your research program with reserved lots. Need vials in bulk? Contact your Fisher Scientific Sales representative for tailored quotes. Tight Deadline? Our cells are available for next day delivery after the purchase order is received. Accelerate Your Research! Try our Attachment Factor (4Z0-201, 4Z0-210) – innovative 5 seconds plating solution for all surfaces.
ATCC Quantitative Genomic DNA from; Bacillus atrophaeus; Strain: NRS 1221A
Quantitative genomic DNA from Bacillus atrophaeus strain NRS 1221A can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.