General Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay EP2 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS185RTA)
The Ready-to-Assay EP2 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER2 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGER2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx KILR T2 Cell Pool, 2 vials. (97-1025P038)
The KILR T2 cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of T2 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.
Eurofins Discoverx KILR WIL2-S Cell Pool, 2 vials. (97-1013P027)
The KILR WIL2-S cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of WIL2-S cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.
Eurofins Discoverx ChemiBrite FSH Glycoprotein Hormone Receptor Stable Cell Line, 2 vials. (HTS178L)
The ChemiBrite FSH Glycoprotein Hormone Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. follicle stimulating hormone) based activation of FSHR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FSHR alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FSHR, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen PK2 Prokineticin Receptor Membrane Preparation, 1 vial. (HTS182M)
The ChemiScreen PK2 Prokineticin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of PROKR2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen MRGPRD Receptor Stable Cell Line, 2 vials. (HTS206C)
The ChemiScreen MRGPRD Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. β-alanine) based activation of MRGPRD GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MRGPRD alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MRGPRD, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx CHO-K1 F2RL1 Gq Cell Line, 2 vials. (95-1006C2)
The F2RL1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serine proteases, SLIGRL-NH2) based activation of F2RL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses F2RL1, a naturally Gq coupled GPCR. Upon activation of F2RL1, the resulting calcium activity can be fluorescently assayed using the Calcium No WashPLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PrecisION hKv2.1 Recombinant Stable Cell Line, 2 vials. (CYL3022)
The PrecisION hKv2.1 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv2.1 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv2.1 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx PrecisION hNav1.4 Recombinant Stable Cell Line, 2 vials. (CYL3024)
The PrecisION hNav1.4 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Nav1.4 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Nav1.4 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx Ready-to-Assay GPBA Bile Acid Receptor Frozen Cells, 2 Vials of Cells. (HTS238LRTA)
The Ready-to-Assay GPBA Bile Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. lithocholic acid, other bile acids) based activation of GPBAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GPBAR1 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GPBAR1, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen P2Y4 Purinergic Receptor Stable Cell Line, 2 vials. (HTS211C)
The ChemiScreen P2Y4 Purinergic Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. ATP, UTP) based activation of P2RY4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses P2RY4 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of P2RY4, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen Y4 Neuropeptide Y Receptor Stable Cell Line, 2 vials. (HTS204C)
The ChemiScreen Y4 Neuropeptide Y Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. peptide YY) based activation of NPY4R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NPY4R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of NPY4R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CGRP1 Calcitonin Receptor-Like Receptor Stable Cell Line, 2 vials. (HTS172C)
The ChemiScreen CGRP1 Calcitonin Receptor-Like Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin) based activation of CALCRL - RAMP1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CALCRL alongside RAMP1 modifying protein and endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CALCRL, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen CGRP1 Calcitonin Receptor Membrane Preparation, 1 vial. (HTS172M)
The ChemiScreen CGRP1 Calcitonin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CALCRL surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen OXGR1 α-Ketoglutarate Receptor Stable Cell Line, 2 vials. (HTS191C)
The ChemiScreen OXGR1 α-Ketoglutarate Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-ketoglutarate) based activation of OXGR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OXGR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of OXGR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.