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Leukapheresed CD19 B cells are negatively selected from leukapheresed peripheral blood MNCs using an immunomagnetic labeling system to remove unwanted cell types leaving the B cells untouched Cells are cryopreserved in Lonza Cryo Media in a cryovial Cell purity is determined post thaw using a FACS assay Count and viability is determined using AO/PI Cells are collected from healthy donors following IRB protocols
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