This monoclonal antibody recognizes a unique PEDV antigenic determinant, enabling precise detection in immunoassays. Clone 5B9 delivers strong affinity and low background in ELISA, Western blotting, and antigen‑capture formats. MPEDV‑5A‑5B9 is ideal for diagnostic development, vaccine evaluation, and studies examining PEDV pathogenesis. Its consistent lot‑to‑lot performance ensures reliable detection across diverse sample types.