Rabbit Secondary Antibodies
Secondary antibodies where rabbit is the host species. Secondary antibodies must be raised against the host species of the primary antibody. Includes different antigens, classifications, conjugations, and isotypes.
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| Content And Storage | Alkaline Phosphatase Conjugated Affinity Purified anti-Goat IgG can be stored vial at 4 °C before opening. DO NOT FREEZE. This product is stable at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product. |
|---|---|
| Target Species | Goat |
| Host Species | Rabbit |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Western Blot,Immunohistochemistry |
| Form | Liquid |
| Isotype | IgG |
| Antigen | Rabbit Anti-Goat IgG (H&L) |
| Regulatory Status | RUO |
| Purification Method | Alkaline Phosphatase Conjugated Affinity Purified anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins. |
| Dilution | Alkaline Phosphatase Conjugated Affinity Purified anti-Goat IgG . is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:1,000 - 1:4,000, Western Blot 1:500 - 1:2,000, Immunohistochemistry 1:200 - 1:1,000. (Optimal dilution should be determined by user). |
| Product Type | Antibody |
| Formulation | 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.01% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA) |
| Classification | Polyclonal |
| Primary or Secondary | Secondary |
| Test Specificity | Minimum Cross reactivity to Human Serum Proteins |