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Rabbit Anti-Goat IgG (H&L) Antibody (Alkaline Phosphatase), ProSci
SDP

Catalog No. 16817844 Shop All ProSci Products
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0.5 mg
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16-817-844 0.5 mg
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Catalog No. 16-817-844 Supplier ProSci LLC Supplier No. XR93800500
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Description

Secondary Antibody

Rabbit Anti-Goat IgG (H&L) Antibody specifically detects Goat Anti-Mouse IgG (H&L) in Goat Samples. It is validated for ELISA, Western Blot, Immunohistochemistry.

Specifications

Antigen Rabbit Anti-Goat IgG (H&L)
Applications ELISA, Western Blot, Immunohistochemistry
Classification Polyclonal
Conjugate Alkaline Phosphatase (AP)
Dilution Alkaline Phosphatase Conjugated Affinity Purified anti-Goat IgG . is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:1,000 - 1:4,000, Western Blot 1:500 - 1:2,000, Immunohistochemistry 1:200 - 1:1,000. (Optimal dilution should be determined by user).
Formulation 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.01% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA)
Host Species Rabbit
Purification Method Alkaline Phosphatase Conjugated Affinity Purified anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Quantity 0.5 mg
Regulatory Status RUO
Primary or Secondary Secondary
Test Specificity Minimum Cross reactivity to Human Serum Proteins
Target Species Goat
Content And Storage Alkaline Phosphatase Conjugated Affinity Purified anti-Goat IgG can be stored vial at 4 °C before opening. DO NOT FREEZE. This product is stable at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Product Type Antibody
Form Liquid
Isotype IgG
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