Hyper Assembly Cloning Kit is designed for fast, directional cloning of one or more target fragment of DNA into any vector. This method employs the 3-5 exonuclease activity of Hyper Assembly Enzyme to get 15-20nt complementary arms at the ends of PCR-generated inserts and linearized vectors, then fuse the DNA fragments to get the recombinant vectors. These 15-20-bp overlaps can be engineered by designing primers for amplification of the desired sequences. Clone any insert into any location within any vector you choose Efficiently clone a broad range of fragment sizes Clone multiple DNA fragments simultaneously into any vector in a single reaction No restriction digestion, phosphatase treatment, or ligation required Other sizes are also available. Please inqury us for quote.