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PCR cloning kits contain reagents and components for inserting PCR-amplified DNA fragments into cloning vectors. Used in molecular biology laboratories, kits may include vectors, enzymes, buffers, primers, control DNA, and competent cells.
A σ1 receptor antagonist (Ki = 0.01 nM); selective for σ1 receptor over σ2 receptor and DAT (Kis = 295.12 and 1,585 nM, respectively); decreases proliferation of MCF-7 and MCF-7/adr cancer cells at 10 µM; induces cell cycle arrest at the G0/G1 phase in MCF-7 and MCF-7/adr cancer cells and apoptosis in MCF-7/adr cancer cells at 10 µM; increases the duration of morphine-induced latency to tail withdrawal in the tail-flick test in mice at 1 mg/kg 30 minutes prior to the test; reduces stress-induced food consumption in rats at 3 or 7 mg/kg
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The LR Cloning Enzyme Kit promotes in vitro recombination between attL- and attR-flanked regions on entry clones and destination vectors to generate attB-containing expression clones.
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This push driven trolley is designed for efficient material handling, offering a robust load capacity and adaptable flange width. It provides reliable movement for various industrial applications.
Push driven for versatile material transport.
Supports loads up to 4000 lb.
Adjustable to accommodate flange widths from 2.6 to 8.67 in.
Large wheel diameter for smooth operation.
Hassle-free guarantee ensures product satisfaction and easy returns within 30 days.
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Intact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step. Cloning takes usually 10-30 min. with more than 95% cloning efficiency.Applications Clone any insert at any site within any vector Restriction enzyme and phosphatase free system Joining multiple large fragments at once Precise insertion at a desired orientation Rapid and high efficiency with > 95% positive clones
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