Transfection Reagents
Agilent Technologies XL1-BLUE ELECTROCOMP CELLS
XL1-Blue Electroporation-Competent Cells are optimal hosts for propagating both lambda and plasmid vectors. They are endA minus cells, Improve the quality of miniprep DNA, allow for color screening and single-strand rescue of phagemid DNA
Agilent Technologies XL2-BLUE MRF ULTRACOMP CELLS
XL2-Blue MRF' Ultracompetent Cells deficient in all known E. coli K12 restriction systems. Increase the efficiency of introducing eukaryotic DNA into E. coli, mcrA, mcrCB and mrr mutations, prevent cleavage of methylated DNA.
Sigma Aldrich Fine Chemicals Biosciences X tremeGENETM siRNA Transf
Short interfering RNA (siRNA) can be directlyintroduced intocells by transfection. A lipid-based transfection reagent such as X-tremeGENE siRNA Transfection reagent can provide a convenient reliable and efficient vehicle for delivering siRNAs into animal cells enabling the study of cellular and functional consequences of gene knockdown.This innovative reagent forms a complex with siRNA as well as with mixtures of siRNA and plasmid DNA (cotransfection) and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps mix and incubate diluted transfection reagent with diluted siRNA then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity it can be used without media changes. The product is animal-component free.ContentsSOLUTIONon filtered through 0.2 mum pore size membrane supplied in polypropy
Kyfora Bio PEI MAX - Transfection Grade (Linear Polyethylenimine Hydrochloride MW 40,000) 1GR
Kyfora Bio’s Polyethyleneimine MAX (PEI MAX) is a powerful, reliable, and cost-effective reagent, widely regarded as the gold standard for both in vitro and in vivo applications. PEI MAX can be used to produce high viral, recombinant protein, and monoclonal antibody titers across a diverse array of cell lines, including HEK293 and CHO cells. It offers exceptional transfection efficiency for both stable and transient transfections in adherent and suspension cultures, all while preserving cell viability, making it an ideal transfection reagent compared to other products on the market.
Agilent Technologies GIGAPACK III XL PACK EXTRACT
Gigapack III XL packaging extract is used to package recombinant lambda phage with selectivity for phage containing large inserts. Preferentially packages phage with large inserts; For genomic lambda and cosmid libraries
Agilent Technologies SURE COMPETENT CELLS
SURE Competent Cells. For routine cloning of DNA with secondary structures, > /= 5 x 108 transformants/microgram. Restriction-Minus Competent Cells for Most Eukaryotic DNA. F- derivative for BAC cloning.
Lonza Walkersville P3 Primary Cell 4D-Nucleofector X Kit L
The P3 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2 x 0.675 mL P3 Primary Cell Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessels12 x Single use pipettes
Lonza Walkersville SE Cell Line 4D-Nucleofector X Kit S
The SE Cell Line 4D-Nucleofector X Kit S is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well Nucleocuvette Strip format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsMedium throughput applicationsOptimization of transfection conditions e.g. substrate titrationsContent:1 x 0.675 mL SE Cell Line Nucleofector Solution1 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0),2 x 16-well Nucleocuvette Strips (20 μL)
Sciencell Research Laboratories RAT EPIDRMAL KERATINOCYTES 1ML
NC3451200 RAT EPIDRMAL KERATINOCYTES 1ML
EDITCO BIO INC Transfection Optimization Kit (Multi-guide)
The Transfection Optimization Kit is a validated positive control kit based on Synthego’s multi-guide strategy that targets human TRAC gene. Recommend using this kit to optimize transfection conditions for your cell type prior to using the Gene Knockout Kit v2. Consider a cell line has been optimized when you achieve at least 50% editing efficiency for TRAC positive control.
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 6 x 0.2 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Agilent Technologies GENEJAMMER TRANSFECT REAGENT
GeneJammer Transfection Reagent 1mL 1.33 mg/mL reagent in 80% ethanol Applications Incorporation of foreign genes into mammalian cells including primary cultures and hard-to-transfect cell lines Transfects DNA