Transfection Reagents
Sigma Aldrich Fine Chemicals Biosciences DOTAP Chloride | 132172-61-3 | MFCD01310872 | 50mg
DOTAP Chloride | Purity: 98% | Mol Wt: 698.54 | 132172-61-3 | MFCD01310872 | 50mg
Genesee Scientific Corporation PEIPRO 1.5 ML
PEIpro is an optimized PEI-based transfection reagent for scalable virus production
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO GREEN INDICATOR, 20NMOL
siGLO Green transfection indicator,20nmol
Lonza Walkersville Human B Cell Nucleofector Kit
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human B cells.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human B Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Human Monocyte Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human monocytes.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Monocytes0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Human Macrophage Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human macrophages.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Macrophages0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Fibroblasts
In addition to various cell type-specific kits for primary fibroblasts, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian fibroblast type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for fibroblasts.Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Transfection efficiencies up to 90%Viabilities up to 98%Applications:Kit suited for fibroblasts from different mammalian species and various organs Already tested for macaque dermal fibroblasts bovine fibroblasts human colon myofibroblasts mouse lung fibroblasts etc.
Lonza Walkersville Cell Line Optimization Nucleofector Kit for Nucleofector Device
The Cell Line Optimization Nucleofector Kit is the ideal tool to determine the optimal transfection conditions for any difficult-to-transfect cell line that lacks an Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). It enables you to conveniently determine the optimal Nucleofection condition of your cell line of interest within one experiment. The kit contains two different Cell Line Nucleofector Solutions, V and L, each of which should be tested in combination with seven different Nucleofector Programs. It is suitable for 18 optimization reactions. Fine-tuning for optimal results can then be performed together with our Scientific Support Team.Benefits:Efficient transfection of virtually any difficult-to-transfect cell lineSimple and rapid optimization completed within just one experimentApplications:Optimization of Nucleofection conditions of virtually any difficult-to-transfect cell line with the Nucleofector II/2b Device
Santa Cruz Biotechnology SIRNA TRANSFECTION REAGENT
siRNA Transfection Reagent, Liquid, Clear, Stable, Soluble in water, Extremely hazardous: in case of inhalation, in case of skin contact, in case of eye contact, May be fatal if swallowed, Storage: 4degreeC, Keep container tightly closed
Apexbio Technology LLC ARCA EGFP mRNA, 100ug (1mg/mL). Cas: MFCD: NA. Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells.
EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence once has been transfected into cells, which makes it always used as a direct detection reporter mRNA. You can observe the fluorescence at 509 nm. Our product had already been modified with ARCA with a high efficient co-transcriptional capping method, which means most of mRNA has a Cap 0 structure. It is a more mature mRNA than mRNA with no capping and maintain a more stable state which lead to higher expressing efficiency in transfected mammalian cells. ARCA obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP. Other sizes are available. Please inqury us for quote.
