Transfection Reagents
ATCC HepAD38 (HepG2 Derivative) Human Liver Cell Line, Inducible HBV Expression, Adherent, BSL-2, ~4.5x10^6 Cells/Vial
HepAD38 is a human, liver-derived HepG2 derivative engineered for tetracycline-regulated (inducible) expression of the hepatitis B virus genome. It is used for antiviral compound screening and for production of encapsidated HBV DNA when expression is induced.
- Inducibly expresses the hepatitis B virus genome under tetracycline control.
- Suitable for antiviral screening and HBV DNA production.
- Adherent, epithelial-like cells derived from human liver.
- Maintain in DMEM/F12 with 10% fetal bovine serum; include tetracycline to repress expression.
- Typical vial contains approximately 4.5 x 10^6 cells; store in vapor-phase liquid nitrogen.
- Biosafety level 2 handling is recommended.
- Culture at 37°C in 95% air, 5% CO2.
Sigma Aldrich Fine Chemicals Biosciences Hexadimethrine bromide, 28728-55-4, MFCD00133397, 5g
≥94% (titration), Synonym: 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide, Polybrene
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO GREEN INDICATOR, 20NMOL
siGLO Green transfection indicator,20nmol
Supply Solutions Roche - X-tremeGENE HP DNA Transf. Reag. 0.4 ml; High-performance polymer reagent for transfecting many cell lines; pkg of 0.4 mL (06366244001) pkg of 1.0mL (06366236001) pkg of 5 x 1 mL (06366546001)
Roche - X-tremeGENE HP DNA Transf. Reag. 0.4 ml; High-performance polymer reagent for transfecting many cell lines; pkg of 0.4 mL (06366244001) pkg of 1.0mL (06366236001) pkg of 5 x 1 mL (06366546001); Hazardous: Y
HORIZON DISCOVERY BIOSCIENCES LIMITED siGENOME Non-Targeting Control siRNAs #2 | 5nmol
siGENOME Non-Targeting Control siRNAs are highly functional, chemically synthesized, and mostly unmodified negative control siRNA duplexes for experiments in human, mouse, and rat cells. siGENOME Non-siGENOME Non-Targeting siRNA #2 targets firefly luciferase mRNA (U47296). These siRNAs can be used either as a non-targeting siRNA in human, mouse or rat cell lines or as a positive silencing control in systems using firefly luciferase as a reporter gene (pGL3 cloning vector, Promega). Targeting Controls were designed to have a minimum of 4 mismatches to all human, mouse and rat genes, and confirmed to have minimal targeting by genomewide microarray analysis. Changes in mRNA or protein levels in cells treated with these controls reflect a baseline cellular response that can be compared to the levels in cells treated with target-specific siRNA.
Sigma Aldrich Fine Chemicals Biosciences NeuroPorterTM Transfection
Neuroporter(TM) Transfection Reagent is a unique formulation of a proprietary cationic lipid optimized for delivery of DNA into primary neurons glial cells and cultured neuronal cell lines with high efficiency and low toxicity. The Neuroporter(TM) Transfection Kit was designed for difficult-to-transfect primary neurons addressing past problems such as poor cell viability low transfection efficiency and neuro-degeneration.
Amsbio LLC GenePORTER Transfection reagent
The GenePORTER Reagent is the original transfection lipid developed by Genlantis containing a unique formulation of the neutral lipid dioleoyl phosphatidylethanolamine (DOPE) and using the proprietary and innovative Direct Hydrophilic Conjugation (DHC) technology. The GenePORTER reagent is optimized for serum-free transfection conditions in a wide variety of commonly used cells. The GenePORTER reagent is especially effective in Jurkat cells and gives the highest transfection efficiencies relative to all other commercial reagents.
DI TNC1 Rat Type 1 Astrocyte Cell Line (ATCC CRL-2005)
DI TNC1 (ATCC CRL-2005) is an immortalized rat type 1 astrocyte cell line established from primary cultures of diencephalon tissue of 1-day-old rats. Cells were transfected with an SV40 early region construct under the GFAP promoter and retain type 1 astrocyte characteristics such as GFAP immunoreactivity and high-affinity GABA uptake.
- Established from diencephalon tissue of 1-day-old rat neonates.
- Immortalized via SV40 early region under GFAP promoter.
- Retains GFAP immunoreactivity and type 1 astrocyte phenotype.
- High-affinity GABA uptake inhibitable by beta-alanine.
- Adherent growth suitable for monolayer culture.
- Culture in Dulbecco's Modified Eagle's Medium with 10% fetal bovine serum at 37°C and 5% CO2.
- Cryopreserve in medium with 5% dimethyl sulfoxide; store in liquid nitrogen vapor.
- Mycoplasma not detected in tested stocks.
Genesee Scientific Corporation POLYPLUS-TRANSFECTION 10100002
NC3672959 POLYPLUS-TRANSFECTION 10100002