Transfection Reagents
Lonza Walkersville Primary Cell Optimization 4D-Nucleofector X Kit
The Primary Cell Optimization 4D-Nucleofector X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector X Unit.Applications:Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector X UnitContent:0.675 mL Nucleofector Solution P10.675 mL Nucleofector Solution P20.675 mL Nucleofector Solution P30.675 mL Nucleofector Solution P40.675 mL Nucleofector Solution P54 x 0.15 mL Supplement 11 x 0.15 mL Supplement 350 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)6 x 20 µL 16-well Nucleocuvette Strips
Bioworld BioFectin™, 0.5 mL
BioFectinTMis a newly developed second generation of lipid-based Transfection Reagent that is optimized for transfection in normal serum-containing medium reagents.It is a one-step solution for all transfection needs.It is highly effective with DNA and siRNA transfection.Features:- For High efficiency DNA and siRNA transfection- Low toxicity- very broad nucleic acid to reagent ratios, no need to optimize conditions- Works in serum-containing medium Appearance: Liquid
Sigma Aldrich Fine Chemicals Biosciences X-tremeGENETM 360 Transfec
X-tremeGENE(TM) 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility X-tremeGENE(TM) 360 delivers high efficiency transfection in more cell types with low cytotoxicity.
SignaGen Laboratories GENJET REAGENT EPITHELIAL 1ML
GenJet DNA In Vitro Tranfection Reagent for epithelial was pre-optimized for most efficiently transfecting epithelial cells.
Lonza Walkersville P3 Primary Cell 4D-Nucleofector LV Kit L
The P3 Primary Cell 4D-Nucleofector LV Kit L is suited for larger scale transfection of primary cells in the 1 mL Nucleocuvette Cartridge format when working with the 4D-Nucleofector LV Unit. With the 1 mL Nucleocuvette Cartridge a fixed volume of 1 mL can be transfected. The cartridge is manually filled via a sterile injection port.Applications:For large scale transfection of up to 1 x 10⁸ cellsEx-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)Generation of large numbers of transiently modified primary cells for cell-based assaysContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 1 mL Nucleocuvette Cartridge
Andwin Scientific JETPRIME DNA/SIRNA RGNT 5ML
The jetPRIME DNA and siRNA Transfection Reagent is made to guarantee high DNA transfection efficiency and excellent gene silencing. This product is very cost-effective as it uses less reagent and DNA and provides more gentle transfections in comparison to other competitors. It can be used with no difficulty and with serum and antibiotics. This can produce 15 reactions in 6-well plates and has a buffer volume of 5 mL. It is sold individually.
STEMCELL Technologies ReproRNA™-OKSGM, Size: 12 µg
ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1). This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2). When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4). ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6). Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media (mTeSR™1, TeSR™2, or TeSR™-E8™)
SignaGen Laboratories GENJET DNA REAGENT NIH 3T3 1ML
GenJet DNA In Vitro Tranfection Reagent for 3T3 cell was pre-optimized for most efficiently transfecting NIH 3T3 cells.
Supply Solutions Roche - X-tremeGENE 9 DNA Transf. Reag. 1.0 ml; Polymer reagent for transfecting common cell lines; pkg 0.4 mL (06365779001) pkg of 1.0 mL (06365787001) pkg of 5 x 1.0 mL (06365809001)
Roche - X-tremeGENE 9 DNA Transf. Reag. 1.0 ml; Polymer reagent for transfecting common cell lines; pkg 0.4 mL (06365779001) pkg of 1.0 mL (06365787001) pkg of 5 x 1.0 mL (06365809001)
Oz Biosciences Helix-IN Transfection Reagent, 1 mL
Helix-IN™ DNA Transfection Reagent opens up new possibilities for addressing issues of classical transfection technologies.OZ Biosciences revolutionizes Polyfection with the design of Helix-IN™, a novel patented Cationic Hydroxylated Amphiphilic Multi-block Polymer (CHAMP™ Technology). This novel bi-functional co-polymer is biocompatible, ionizable, pH responsive and biodegradable.Cellular stress is reduced, viability preserved and efficiency enhanced.Broad Spectrum transfection Reagent for cell lines and hard-to-transfect cellsHigh Intracellular Protein Production while preserving viabilityHigh secreted Protein Production while minimizing cellular stressBiodegradable - Avoid secondary effectsCompatible with any culture medium
SignaGen Laboratories GENJET DNA REAGENT L929 1ML
GenJet DNA In Vitro Tranfection Reagent for L929 was pre-optimized for most efficiently transfecting L929 cells.
Lonza Walkersville P3 Primary Cell 4D-Nucleofector LV Kit XL
The P3 Primary Cell 4D-Nucleofector LV Kit XL* is suited for larger scale transfection of primary cells in the LV Nucleocuvette Cartridge format when working with the 4D-Nucleofector LV Unit. With the LV Nucleocuvette Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.Applications:For large scale transfection of up to 1 x 10⁹ cellsEx-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)Generation of large numbers of transiently modified primary cells for cell-based assaysContent:22.5 mL P3 Primary Cell Nucleofector Solution5 mL Supplement 1,1 x LV Nucleocuvette Cartridge2 x 4D-Nucleofector LV Reservoir
Mayflower Bioscience LentiBOOST-P Pharma Grade Lentivirus Transduction Enhancer Solution
LentiBOOSTTM is a highly effective, non-cytotoxic transduction enhancer for preclinical and clinical application of lentiviral vectors. As universally acting (receptor independent) adjuvant it can be applied to a wide range of clinically relevant cell types including CD34+ hematopoietic stem cells (HSCs), primary T cells and NK cells. These unique features make it a promising candidate to improve clinical transduction protocols for ex vivo gene therapies and CAR-T cell therapies.
Andwin Scientific INTERFERIN SIRNA TRANSFECT 1ML
The INTERFERin siRNA Transfection Reagent guarantee reproducible transfection, and attain over 90% silencing in a broad variety of cells. A 1 milliliter volume of this reagent is enough to perform 500 to 1000 transfections in 24-well plates. This product can be used with serum and antibiotics. It must be stored at 4C (39F). This product is sold individually in a quantity of 1 mL.
Lonza Walkersville Human Macrophage Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human macrophages.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Macrophages0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes