Transfection Reagents
Lonza Walkersville Cell Line Nucleofector Kit C
The Cell Line Nucleofector Kit C is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A2058CCRF-CEMMDA-MB-453MEG-01MG-63NCI-H1299 [H1299]NS0 (ECACC)SK-BR-3T2U266B1U-937The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution C0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT 2 TRANSFECTION RGNT
DharmaFECT 2 Transfection Reagent
Lonza Walkersville Human Stem Cell Nucleofector Kit 1
The Nucleofector 2b Device (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector Kit 1 is one of our two kits suited for efficient transfection of human stem cells. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Stem Cells0.5 mL Supplement 1, 30 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
FENICSBIO CORP NCI-H929/GFP STABLE CELL LINE
NCI-H929/GFP cells are derived from the human B lymphocyte NCI-H929 cell line by stably integration of a constitutive GFP stably expression construct. NCI-H929 cells stably express B cell maturation antigen (BCMA)-a novel treatment target for multiple myeloma (MM), and have been widely used in cancer research and drug development. NCI-H929/GFP cells stably stably expressGFP, can be used for in vitro assays and in vivo imaging.
Sigma Aldrich Fine Chemicals Biosciences REAGENT KIT FOR MRP2 MRP3 MRP5
NC3883695 REAGENT KIT FOR MRP2 MRP3 MRP5
LGC GENOMICS LLC (Lucigen) TransforMax EC100D pir-116 Electrocompetent E. coli, 10 RxnsPerform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
These cells are used for rescue cloning of DNA transposed using the EZ-Tn5™
Sigma Aldrich Fine Chemicals Biosciences Hexadimethrine bromide | 28728-55-4 | MFCD00133397 | 50g
Hexadimethrine bromide | 94% | 28728-55-4 | MFCD00133397 | 50g
Lonza Walkersville Cell Line Nucleofector Kit L
The Cell Line Nucleofector Kit L is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device.This kit has been determined to be optimal for transfecting following cell types:3T3-L1 adA-10BHK-21BxPC-3DU145EL4FDC-P1GH3 H9c2 (2-1)HeLa S3IMR-32KG-1aL-428 (DSMZ)MDCKMDCK II (ECACC)MOLT-4MV-4-11NTERA-2 cl.D1WEHI-231The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format