Transfection Reagents

ATCC CF52.Tr (CRL-6244) Canine Adherent Fibroblast Cell Line, BSL-1

CF52.Tr (CRL-6244) is a canine fibroblast adherent cell line from ATCC's NBL Collection supplied for in vitro research applications. The line is maintained under standard mammalian culture conditions and handled at biosafety level 1.

• Derived from Canis familiaris (dog).
• Fibroblast cell type with adherent growth properties.
• Recommended medium: DMEM with 4 mM L-glutamine and 10% fetal bovine serum.
• Incubation: 37°C in a 5% CO2 atmosphere.
• Biosafety level 1 handling.
• Part of the NBL Collection and fully accessioned for research use.

MDA-T68 (ATCC CRL-3353) Human Papillary Thyroid Carcinoma Cell Line, Frozen Vial (~2.0 x 10^6 cells, 1.0 mL)

MDA-T68 (CRL-3353) is a human epithelial-like cell line derived from thyroid tissue of a 75-year-old male with the follicular variant of papillary thyroid carcinoma. It is intended for in vitro research applications such as therapeutic testing and molecular studies; the line harbors HRAS Q61K and TERT promoter -124 C>T mutations and shows detectable TTF-1 expression.

• Adherent growth with epithelial morphology.
• Population doubling time approximately 45 hours.
• Supplied frozen in a 1.0 mL vial with approximately 2.0 x 10^6 cells.
• Recommended medium: RPMI-1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, and 0.1 mM nonessential amino acids.
• Culture conditions: 37°C, 95% air/5% CO2; maintain 5 x 10^4 to 2 x 10^5 cells/cm^2.
• Subculture ratio 1:3 to 1:8; renew medium 2-3 times per week.
• Store long term in the vapor phase of liquid nitrogen; handle thawed cultures under aseptic conditions.