Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, >=4,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU
Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, >=4,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 2KU
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white, BioUltra, lyophilized powder, ≥40,000 units/mg protein | 12650-88-3 | MFCD00131557 | 5g
Lysozyme from chicken egg white, BioUltra, lyophilized powder, ≥40,000 units/mg protein | Purity: 0.98MW: | 12650-88-3 | MFCD00131557 | 5g
Sigma Aldrich Fine Chemicals Biosciences beta-Glucuronidase from Helix pomatia Type H-2, aqueous solution, >=85,000 units/mL | 9001-45-0 | MFCD00130629 | 25ML
beta-Glucuronidase from Helix pomatia Type H-2, aqueous solution, >=85,000 units/mL | 9001-45-0 | MFCD00130629 | 25ML
Sigma Aldrich Fine Chemicals Biosciences Diaphorase from Clostridium kluyveri lyophilized powder, 3.0-20.0 units/mg protein (biuret) | 9001-18-7 | MFCD00130947 | 1.5KU
Diaphorase from Clostridium kluyveri lyophilized powder, 3.0-20.0 units/mg protein (biuret) | 9001-18-7 | MFCD00130947 | 1.5KU
Sigma Aldrich Fine Chemicals Biosciences Deoxyribonuclease I from bovine pancreas | 9003-98-9 | MFCD00130918 | 10 mg
Deoxyribonuclease I from bovine pancreas | Mol Wt: ~31 kDa | 9003-98-9 | MFCD00130918 | 10 mg
Sigma Aldrich Fine Chemicals Biosciences Alcohol Oxidase solution from Pichia pastoris | 9073-63-6 | MFCD00130452 | 250 units
Alcohol Oxidase solution from Pichia pastoris | 9073-63-6 | MFCD00130452 | 250 units
Sigma Aldrich Fine Chemicals Biosciences Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, lyophilized powder, >=550 units/mg protein (biuret) | 9001-40-5 | MFCD00081656 | 200UN
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, lyophilized powder, >=550 units/mg protein (biuret) | 9001-40-5 | MFCD00081656 | 200UN
Sigma Aldrich Fine Chemicals Biosciences Catalase from Aspergillus niger ammonium sulfate suspension, >=4,000 units/mg protein | 9001-05-2 | MFCD00081483 | 10MG
Catalase from Aspergillus niger ammonium sulfate suspension, >=4,000 units/mg protein | 9001-05-2 | MFCD00081483 | 10MG
Sigma Aldrich Fine Chemicals Biosciences Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 7.5 KU
Alcohol Dehydrogenase from Saccharomyces cerevisiae | Mol Wt: ~141,000 | 9031-72-5 | MFCD00081305 | 7.5 KU
Sigma Aldrich Fine Chemicals Biosciences alpha-Amylase from human saliva Type XIII-A, lyophilized powder, 300-1,500 units/mg protein | 9000-90-2 | MFCD00081319 | 5KU
alpha-Amylase from human saliva Type XIII-A, lyophilized powder, 300-1,500 units/mg protein | 9000-90-2 | MFCD00081319 | 5KU
Kactus TelN Protelomerase (TLN-BE001) 10KU
TelN Protelomerase is cloned from bacteriophage N15. TelN cuts dsDNA at the recognition site TelRL (56 bp), which consists of a palindromic sequence-TelO in the middle and palindromic sequences R3 and L3 at both ends of 14 bp form. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini.
INTACT GENOMICS INC T7 Endonuclease I, 1,250 Units
T7 Endonuclease I is a stable homodimer of identical 149 amino acid subunits, produced from a recombinant gene in *E. coli*. It recognizes and cleaves mismatched DNA where the mismatched DNA length position is more than 1 bp. This enzyme is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells, which are generated by CRISPR/ZFN/TALEN at desired target sites, PCR-amplified, denatured, and re-annealed to form mismatched DNA.
- Resolves four-way junction or branched DNA.
- Detects or cleaves heteroduplex and nicked DNA.
- Randomly cleaves linear DNA for shot-gun cloning.
- Physical purity is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining.
- Includes T7 Endonuclease I and 10x T7 Endonuclease I reaction buffer.
- Storage temperature: -20°C.
- Free from detectable contaminating nuclease activities.
Gold Biotechnology Inc Proteinase K Solution 20 mg/ml 2 mL
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.
