Modifying Enzymes
Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Invitrogen™ Ambion™ RNase H, from E. coli, 10 U/μL
Endonuclease-free, exonuclease-free, protease-free
Invitrogen™ DNase I, Amplification Grade
Purified and tested for nondetectable levels of RNase contamination
Invitrogen™ Ambion™ Recombinant RNase A
Rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Fisher BioReagents™ Ribonuclease A (From Bovine Pancreas)
Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
MilliporeSigma™ Calbiochem™ β1-2,3,4,6-N-Acetylglucosaminidase
Catalyzes hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates
MP Biomedicals™ Dispase
From Bacillus polymyxa
MilliporeSigma™ Novagen™ rLysozyme™ Solution
For lysis of Gram-negative bacteria