Modifying Enzymes
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Invitrogen™ DNA-free™ DNA Removal Kit
Safely eliminate DNA contamination from RNA samples
Thermo Scientific™ RNase A/T1 Mix
Get the RNA degradation activity of both RNase A and RNase T1 with Thermo Scientific™ RNase A/T1 Mix.
Invitrogen™ Ribonuclease H
Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA
Invitrogen™ AcTEV™ Protease
Specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), ideal for removing affinity tags from fusion proteins
Thermo Scientific™ T4 beta-glucosyltransferase
Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis
Invitrogen™ LR Clonase™ II Plus enzyme
Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes.
Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.