Modifying Enzymes
Apexbio Technology LLC DNase I (RNase-free),1000U
DNase I (RNase-free) is an endonuclease that digests single- or double- stranded DNA, generating dinucleotide, trinucleotide, and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. The activity of DNase I (RNase-free) dependent on Ca2+ and can be activated by Mg2+ or Mn2+. In the presence of Mg2+, DNase I (RNase-free) can randomly cleaves arbitrary sites of double-stranded DNA; in the presence of Mn2+, DNase I (RNase-free) can simultaneously recognize both strands of DNA and cleaves at nearly same sites. DNase I (RNase-free) can act on single stranded DNA, double stranded DNA, chromatin, and RNA: DNA hybrid strands. DNase I (RNase-free) is suitable for RNA extraction, in vitro transcription, and removal of DNA for RT-PCR experiments.Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC DNase I (RNase-free) 10000U
DNase I (RNase-free) is an endodeoxyribonuclease capable of hydrolyzing single-stranded or double-stranded DNA producing dinucleotide trinucleotide and oligonucleotide fragments with 3 -OH and 5 -phosphate ends The activity of DNase I (RNase-free) depends on Ca2 and can be activated by Mg2 or Mn2 In the presence of Mg2 DNase I (RNase-free) randomly cleaves double-stranded DNA at any site in the presence of Mn2 it simultaneously recognizes both DNA strands and cleaves at nearly identical sites DNase I (RNase-free) acts on single-stranded DNA double-stranded DNA chromatin and RNA DNA hybrids In molecular biology experiments DNase I (RNase-free) is suitable for the removal of DNA in RNA extraction in vitro transcription and RT-PCR applications
New England Biolabs, Inc. Induro Reverse Transcriptase - 4000 units
Induro Reverse Transcriptase is a group II intron-encoded RT that exhibits increased thermostability high processivity and increased tolerance of inhibitors in the synthesis of cDNA from RNA. It is an ideal enzyme for challenging cDNA synthesis reactions from long transcripts RNAs with strong secondary structures and RNA samples with inhibitors. Additionally Induro Reverse Transcriptase supports RNA-seq applications such as long-read RNA sequencing.
Oxyrase Oxyfluor 5Ml Bottle 1/Pk
Formulated specifically for fluorescence microscopy. Prevents photo-bleaching and photo-damage by removing dissolved oxygen. Significantly reduces/eliminates formation of oxygen free radicals. Results in brighter cell images. Provides protective effect for biological material being studied. Excellent solution for live cell imaging and cell culture applications.
BIOSS KD-Validated Topoisomerase II
DNA Topoisomerase II Alpha Primary Antibody Unconjugated Public Immunogen Range 362-400/400 Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB FCM IC Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
BIOSS KD-Validated Coenzyme A syntha
Coenzyme A Synthase Primary Antibody Unconjugated Public Immunogen Range 530-564/564 Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB FCM IC Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
New England Biolabs, Inc. EnGen Spy Cas9 HF1 - 2500 pmol
EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage. Spy Cas9 is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The single guide RNA (sgRNA) targets Cas9 to the region immediately upstream of a 5'-NGG-3' protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of the PAM. EnGen Spy Cas9 HF1 contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-termini of the protein.
Ubpbio Recombinant human UbE1 from SF9 insect cells, no tag
UBE1 is an E1 enzyme within the E1, E2, E3 cascade which conjugates Ub to protein substrates. UBE1 activates Ub by catalyzing an ATP – dependent reaction; Ub is conjugated onto the catalytic cysteine residue of UBE1 by formation of a thioester bond. The activated Ub is then passed on to a Ub-conjugating enzyme E2; Ub charged E2 binds a Ub ligase E3 that directly binds substrate proteins. Ub is transferred to the HECT domain E3s prior to being conjugated on substrate proteins. For RING domain E3s, Ub is transferred directly from an E2 to substrate proteins.
Ubpbio N-terminally 6xHis-tagged recombinant human UbE2R1, a ubiquitin conjugating enzyme catalyzing formation of K48 polyubiquitin chains
Ube2R1 (UbcH3) is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Ube2R1 was first discovered during screening for cell cycle regulatory mutations that occur in yeast. While Ube2R1 is able to create diubiquitin chains by itself, it works far more efficiently and effectively with the E3 ligase Skp1 (S- phase kinase- associated protein 1)/cullin/F-box, also known as SCF, to promote substrate polyubiquitination.
Ubpbio N-terminally 6xHis-tagged recombinant human UbC13/UEV1A (UbE2N), a ubiquitin conjugating enzyme catalyzing formation of K63 polyubiquitin chains
UbE2N (Ubc13/UEV1A) is a complex that acts as an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Uev1a must interact with Ubc13 because it lacks the active site that other E2s posses. Ube2N has been known to catalyze K63 – linked Ub chains which aides in activation of kinases in NF – kappaB signaling pathway, amongst other tasks.
New England Biolabs, Inc. Bacteroides Heparinase I – 600 units
Bacteroides Heparinase I, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
Worthington Biochemical Corporation HEPATOCYTE ISOLATION
A component of the Hepatocyte and Papain Dissociation Systems. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease.
TOPOGEN INC HMN RECOMB TOPOISOMERASE 500
Recombinant Human Topoisomerase I (overexpressed in baculovirus). 500 units. Storage: -20 C