Modifying Enzymes
New England Biolabs, Inc. α1-2,4,6 Fucosidase O - 80 units
a1-2,4,6 Fucosidase O is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal a1-2, a1-4 and a1-6 linked fucose residues from oligosaccharides. a1-2,4,6 Fucosidase O cleaves a1-6 fucose residues more efficiently than other linkages.
New England Biolabs, Inc. Antarctic Thermolabile UDG – 100 units
Antarctic Thermolabile UDG (Uracil- DNA Glycosylase) catalyzes the release of free uracil from uracil-containing single-stranded or double-stranded DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperature and high pH. This enzyme is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50C.
Sigma Aldrich Fine Chemicals Biosciences Hyaluronidase from sheep testes Type II, lyophilized powder, >=300 units/mg solid | 37326-33-3 | MFCD00131351 | 100MG
Hyaluronidase from sheep testes Type II, lyophilized powder, >=300 units/mg solid | 37326-33-3 | MFCD00131351 | 100MG
New England Biolabs, Inc. Endoproteinase LysC – 20 µg
Endoproteinase LysC is a serine Endoproteinase which cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.
New England Biolabs, Inc. T3 DNA Ligase – 750000 units
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It will catalyze the formation of a phosphodiester bond between adjacent 5 phosphate and 3 hydroxyl groups of duplex DNA. Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase. As with T4 DNA Ligase, blunt end ligation is enhanced by the addition of PEG 6000 to the reaction. T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology protocols requiring DNA ligase activity.
New England Biolabs, Inc. GpC Methyltransferase (M.CviPI) – 200 units
Methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5´...GC...3´.
Sigma Aldrich Fine Chemicals Biosciences Lysozyme from chicken egg white BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein | 12650-88-3 | MFCD00131557 | 1g
Lysozyme from chicken egg white BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein | 12650-88-3 | MFCD00131557 | 1g
Sigma Aldrich Fine Chemicals Biosciences Carbonic Anhydrase Isozyme II from bovine erythrocytes lyophilized powder, >=3,000 W-A units/mg protein | 9001-03-0 | MFCD00081467 | 25MG
Carbonic Anhydrase Isozyme II from bovine erythrocytes lyophilized powder, >=3,000 W-A units/mg protein | 9001-03-0 | MFCD00081467 | 25MG
Worthington Biochemical Corporation HEPATOCYTE ISOLATION
A component of the Hepatocyte and Papain Dissociation Systems. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease.
New England Biolabs, Inc. Hi-T4™ DNA Ligase – 20000 units
A thermotolerant variant of T4 DNA Ligase, Hi-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher temperatures than wild type T4 DNA Ligase. Hi-T4 DNA Ligase will join blunt end and cohesive end termini as well as repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids at temperatures as high as 50C.
New England Biolabs, Inc. Salt-T4 DNA Ligase – 100000 units
A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase. This enzyme will join cohesive end termini at salt concentrations as high as 300 mM without any loss in activity. This enzyme is insensitive to salt carried over from other reaction components (vector or insert DNA) and allows ligation reactions to proceed in alternative reaction buffers with higher levels of salt (e.g., NEBuffer r3.1).
New England Biolabs, Inc. Tth Argonaute (TtAgo) – 50 pmol
TtAgo is a prokaryotic argonaute from the Gram-negative, thermophilic bacterium Thermus thermophilus which functions as a DNA-guided endonuclease when provided with a 16-18 nucleotide long 5-phosphorylated single-stranded DNA oligonucleotide guide. The RNase H-like active site of TtAgo requires divalent Mg2+ (or Mn2+) metal ions for the reaction to occur and cleaves a complementary substrate between the bases corresponding to positions 10 and 11 of the DNA guide.
Zymo Research Corporation 5-hmC Glucosyltransferase, 100 U
Glucosylation of 5-hydroxymethylcytosine by 5hmC Glucosyltransferase can be used for sequence specific (see Cat. Nos. D5410 & D5411), locus specific, as well as global quantification of 5- hydroxymethylcytosine.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3999297 ENTEROKINASE ENTEROPEPTIDASE