Clone 2E4 is a high‑affinity mouse monoclonal antibody targeting a distinct epitope on C. difficile Toxin A. It provides sensitive, specific detection in ELISA, Western blotting, and antigen‑capture formats, supporting toxin‑monitoring studies, diagnostic development, and research on virulence mechanisms. Its clean signal and low nonspecific binding make it ideal for assays requiring high analytical precision. MACD‑55A‑2E4 offers reproducible performance across repeated runs and diverse sample types.