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Chromogenic assays yield a colored reaction product that absorbs light in the visible range. They are commonly employed in testing for platelet function defects and other coagulation disorders.
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3,3’-Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol and xylene. This product comes in a two-component system consisting of a liquid stable DAB chromogen and DAB substrate buffer. The color of the chromogen solution can vary from colorless to pale brown.
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