Molecular Biology Reagents and Kits
New England Biolabs, Inc. Endonuclease IV – 5000 units
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5' to the lesion leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5' terminus. The enzyme also has a 3' -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5'-phosphate and phosphate from the 3' end of DNA.
New England Biolabs, Inc. RNase H – 1250 units
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
New England Biolabs, Inc. M13K07 Helper Phage – 1.8 ml
M13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA.
New England Biolabs, Inc. M13mp18 RF I DNA – 10 µg
M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13. This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the -Galactosidase gene. When a fragment of DNA is inserted into one of these sites, the -Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate.
New England Biolabs, Inc. PhiX174 RF II DNA – 150 µg
This commonly used cloning vector is the double-stranded nicked circular form of ΦX174 - relaxed form. Its molecular weight is 3.5 x10^6 Daltons and it is 5,386 base pairs in length. Greater then 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ΦX174 DNA.
New England Biolabs, Inc. pTXB1 Vector – 10 µg
pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
New England Biolabs, Inc. PhiX174 Virion DNA – 250 µg
ΦX174 Virion DNA is the single-stranded viral DNA isolated from bacteriophage ΦX174 am3 cs70. It has a molecular weight of 1.70 x 10^6 daltons and is 5386 bases in length. Greater than 85% of the molecules are circular.
Omega Biotek Inc Mag-Bind Total RNA 96 Kit | 4 x 96 preps
The Mag-Bind Total RNA 96 Kit provides a novel technology for total RNA isolation. This kit allows the rapid and reliable isolation of high-quality total cellular RNA and viral RNA from a wide variety of cells and tissues. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, which improves the removal of contaminants and increases nucleic acid purity. Mag-Bind Total RNA 96 Kit procedure can be fully automated with most robotic workstations.
SignaGen Laboratories AAV-GFP (AAV SEROTYPE 9)
Unit Size: 1 x >30 µL (>1E+13 VG/mL.AAV9-GFP is a pre-packaged rAAV in serotype 9 (with capsid from AAV serotype 9a nd 2xITR from AAV serotype 2) which over-expresses EGFP under CMV promoter. Ready to use format.
Braintree Scientific Accuris Mouse Genotyping Kit, 1 hour, mammalian,80 reactions
Accuris™ 1 Hour Mouse/Mammalian Genotyping Kit DNA extraction and amplification in 1 hour Optimize proprietary lysis buffer Single tube - no organic solvents or clean up procedures Enhanced Accuris Hot Start Polymerase included The Accuris Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR Accuris Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers. **Sold for research purposes only and not for human use**
Innovative Research Inc US Ms C57 Srm 10ml
Innovative Grade US Origin Mouse C57BL6 Serum 10ml - Innovative Grade US Origin Mouse C57BL6 Serum from Innovative Research is manufactured using whole blood collections from type C57BL6 mice. The animals used for this material are of US Origin, ensuring that only the highest-quality material is used for the production of the serum.
MilliporeSigma NUCLEOCLEAN DECONTAMINATION SP
Life Sciences,Immunodetection,NucleoClean Decontamination Spray
Roche Diagnostics MagNA Pure 96 Processing Cartridge, 36 cartridges
MagNA Pure 96 disposables are nuclease free according to current Quality Control procedures. They are manufactured from material that is inert to chemical leaching at low and elevated temperatures, that could interfere with downstream applications.