DNA Vectors
Enzo Life Sciences LPS from E. coli, Serotype O55:B5 (TLRGRADE) (Ready-to-Use), (2ml)
Specific TLR4 activator. Alternative Name: Lipopolysaccharide from E. coli, Serotype O55:B5 S-form. Purity: Absence of detectable protein or DNA contaminants with agonistic TLR activity. Use/Stability: Stable for at least 1 year after receipt when stored at +4°C. Long Term Storage: +4°C
Vector Laboratories Mouse On Mouse (M.O.M.) ImmPRESS HRP (Peroxidase) Polymer Kit
Vector M.O.M. Immunodetection Kits are specifically designed to reduce endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue. This problem can be essentially eliminated by using Vector M.O.M. technology. The result is clear, crisp specific staining of the antigens of interest. This Vector M.O.M. ImmPRESS Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step M.O.M. ImmPRESS Peroxidase Polymer reagent. The highly active peroxidase micropolymers of the ImmPRESS Detection System provide outstanding sensitivity, limit steric interference, and enhance accessibility. Contains sufficient reagents to stain approximately 150 mouse sections. Includes 0.6 ml M.O.M. Mouse Ig Blocking Reagent (concentrate), 30 ml R.T.U. 2.5% Normal Horse Serum, and 15 ml R.T.U. M.O.M. ImmPRESS Anti-Mouse Ig Reagent.
ANTIBODY DESIGN LABS CM13d3
CM13d3 is a helper phage engineered for multivalent phage display. CM13d3 is a derivative of M13KO7 (1) having a wild-type pIII phenotype which allows efficient bacterial transduction but lacking a functional pIII gene thus driving the display of pIII-fusion proteins expressed by the phagemid on the phage head (2). The use of pIII-defective helper phage has been shown to increase display of scFv by a factor up to 100-fold and more (3-5). This preparation contains enough helper phage to superinfect up to 0.5 L (500 ml) of TG1 culture. Other bacterial strains may require different amounts of CM13d3 helper phage.
Boca Scientific Inc Exontrap Cloning Vector pET01, Lyophilized DNA, 5μg
The Exontrap vector pET01 has an intrinsic splicing function that enables selective cloning of exon sequences from large genomic eukaryotic DNA fragments. This new route for the identification of eukaryotic genes does not involve an initial isolation of cellular mRNA, allowing genes that are not transcribed during certain life cycle stages to be identified. This greatly facilitates exon/intron mapping. The Exontrap function is based on a shuttle vector containing prokaryotic and eukaryotic genetic elements for replication in both bacteria and cell cultures. The vector contains a 5´ and 3´ exon separated by a 600 bp intron sequence that has a polylinker for cloning.
Expression Systems BestBac 2.0 V-Cath/ChiA Deleted Baculovirus DNA
Expression Systems popular BestBac Linearized Baculovirus DNA backbone has been engineered to deliver highly efficient recombination with all polyhedron gene locus based transfer vectors.
Unmatched productivity and stability for BEVS
Improved production methodology can reduce need for plaque purification
Produces high titers of 5 E8 to 3 E9 IU/mL of recombinant baculovirus in P1 stock
Generates P1 stock as quickly as Bac-to-Bac and other systems
Each BestBac vial contains enough material for a minimum of five cotransfections
Suitable for other expression systems including BacMam™
BestBac 2.0 is a deletion mutant of BestBac 1.0. The v-cath/chiA deletion removes a potentially detrimental protease and may reduce competition for resources for protein synthesis during late gene expression. The v-cath/chiA deletion can improve the integrity of your expressed protein resulting in a significant increase of functional protein yield.