DNA Vectors
Sigma Aldrich Fine Chemicals Biosciences Cas9 Neomycin Lenti Partic1EA
Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Neomycin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Neo using the convenient ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.
New England Biolabs, Inc. pTXB1 Vector – 10 µg
pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
New England Biolabs, Inc. PhiX174 RF II DNA – 150 µg
This commonly used cloning vector is the double-stranded nicked circular form of ΦX174 - relaxed form. Its molecular weight is 3.5 x10^6 Daltons and it is 5,386 base pairs in length. Greater then 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ΦX174 DNA.
New England Biolabs, Inc. Lambda DNA (dam-) – 1250 µg
Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7). The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-). Therefore, those deoxyadenosine positions which are methylated in a wild type E. coli host are not methylated in the dam- host. This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (dam-) is 48,502 base pairs in length.
New England Biolabs, Inc. M13K07 Helper Phage – 1.8 ml
M13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA.
HORIZON DISCOVERY BIOSCIENCES LIMITED HUMAN KRAS G12V/ LIM1215 CE
NC2198029 HUMAN KRAS G12V/ LIM1215 CE