DNA Vectors
Cellecta Inc CRISPRI DCAS9-KRAB PRDKCCB-CMV
CRISPRi dCas9-KRAB pRDKCCB-CMV-dCas9-KRAB-2A-Blast (virus); Quantity: 1 x 10^6 TU; Shipping Temp: -80C
System Biosciences LLC pGreenFire1-ISRE (EF1α-neo) LentivectorpGreenFire1-ISRE (virus) + EF1α-Neo = >2 x 10^6 IFUs
Study ISRE signal transduction by co-expressing dscGFP and luciferase in response to ISRE activity (includes EF1α-neo selection cassette). With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-ISRE (EF1α-neo) Lentivector co-expresses a destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase from ISRE transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of ISRE-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to ISRE activity. The result is the ability to quantitatively measure ISRE activity by fluorescence and luciferase activity.
