DNA Extraction and Purification
Zymo Research Corporation Quick-DNA Fecal/Soil 96 ™ Kit (2 x 96 Preps)
The Quick-DNA Fecal/Soil Microbe 96 Kit is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
Abcam Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)
Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Biochain Institute Inc SeqPure PCR Purification Kit (5 ml), 5 ml/pk
SeqPure PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Biotium AccuGreen™ Broad Range dsDNA Quantitation Solution (100 assays)
The AccuGreen™ Broad Range dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit fluorometer from Thermo Fisher. The AccuGreen™ quantitation solution is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL. This item is a standalone fluorescent DNA quantitation solution. The use of this product on the Qubit fluorometer requires two standards. The assay has a green fluorescence with an excitation/emission of 500/530 nm (with DNA). This trial size has sufficient reagent for 100 assays. This solution does not come with a DNA standard; see the AccuGreen™ Broad Range Kit (31069), which comes with a calf thymus DNA standard and is equivalent to the Qubit dsDNA BR Assay Kit.
Research Products International Corp EZ DNA Methylation-Gold Kit, 200 Rxns
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Complete bisulfite conversion of DNA in less than 3 hours.
Desulphonation and recovery of bisulfite-treated DNA with a spin column.
Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
Biochain Institute Inc Genomic DNA - Dementia: Brain: Parietal Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation EZ DNA Methlyation-Lightning Kit (10 rxns.)
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process - simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 l. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
Biochain Institute Inc Genomic DNA - Arrhythmia, infarct: Interventricular Septum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Adrenal, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation DNA/RNA Shield Lysis Tube (2 mm) (Tissue) (50 pack)
DNA/RNA Shield Lysis and Collection tubes are designed for the streamlined collection, transport, and processing of biological specimen. They are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Esophagus, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation ZR-96 Oligo Clean & Concentrator (2x96)
The ZR-96 Oligo Clean & Concentrator™ kit features Zymo-Spin plate technology and employs a single-buffer system that allows for efficient oligonucleotide adsorption to the matrix of each well in the Zymo-Spin™I-96 Plate. There is no need for organic denaturants or chloroform. Oligonucleotide is washed and concentrated into a small volume of water ( 10 l). Purified oligonucleotide is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
Norgen Biotek Corp FFPE RNA/DNA PURIFICATION KIT
Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. RNA and DNA are sequentially purified into separate fractions using this kit. The kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in high quality and yield of RNA/DNA. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. RNA is purified without using phenol or chloroform. Purified RNA is of the highest integrity and can be used in a number of downstream applications including real time PCR. Purified genomic DNA is also of the highest quality, and can be used in sequencing.
Biotium AccuGreen™ Broad Range dsDNA Quantitation Kit (100 assays)
The AccuGreen™ Broad Range dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL. The assay has a green fluorescence with an excitation/emission of 500/530 nm (with DNA). This trial size kit has sufficient reagent for 100 assays.