DNA Extraction and Purification
New England Biolabs, Inc. NEBNext dsDNA Fragmentase – 250 reactions
NEBNext dsDNA Fragmentase is an enzyme-based reagent that shears DNA to produce fragments of the desired sizes in a time-dependent manner, for next generation sequencing library preparation protocols Note: NEBNext dsDNA Fragmentase is not part of the NEBNext Ultra II FS (Fragmentation System) reagents.
Biochain Institute Inc Genomic DNA - Multiple Sclerosis Disease: Brain: Pons, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Rectum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Enzo Life Sciences PCR & Gel clean-up kit (200tests)
PCR & Gel clean-up columns use silica membrane technology in a mini spin column format to deliver easy and efficient DNA purification from PCR products, agarose gels, and labeling reactions. Sample material: <400 µl PCR reaction mixture, <400mg agarose gel Fragment size: 50bp - ~20kbp Recovery: 70 - 95% Elution volume: 15 - 50 µl Preparation time: 10 min for 6 preps Binding capacity: 25 µg PCR & Gel clean-up columns have been validated for use with our CGH Labeling Kit, Prod. No ENZ-42671. Assay Time: <10 min.
New England Biolabs, Inc. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® – 24 reactions
The Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities. The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA.
- Get more of what you need, with the highest library yields
- Use to generate high quality libraries from a broad range of input amounts, from 500 pg to 1 µg
- Prepare libraries from ALL your samples, including GC-rich DNA and FFPE DNA samples
- Improve yields and quality for target enrichment applications
Biochain Institute Inc Genomic DNA - Alzheimer's Disease: Brain: Precentral Gyrus, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation Quick-DNA Tissue/Insect Microprep Kit (50 Preps)
The procedure is easy and can be completed in as little as 15 minutes: samples are added directly to a ZR BashingBead™ Lysis Tube (2.0 mm), then rapidly and efficiently lysed by bead, without using organic denaturants or proteinases. The DNA is isolated and purified using our Zymo-Spin™ Technology and is ideal for downstream molecular-based applications including PCR, array, genotyping, etc. The procedure is compatible with mammalian tissues, whole blood,and cultured cells.
Active Motif Nucleosome Preparation Kit
Active Motif's Nucleosome Preparation Kit is designed to prepare mono- and oligonucleosomes starting from cells. Nucleosomes comprise the smallest subunit of chromatin and consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Each nucleosome is separated by a linker of DNA. The Nucleosome Preparation Kit uses an enzymatic cocktail to digest the linker DNA. By adjusting the digestion time, it is possible to control the amount of mononucleosomes or oligonucleosomes extracted. Because isolated nucleosomes are more physiologically relevant than histones alone or synthetic peptides, they are ideally suited for use as substrates in the analysis of histone post-translational modifications, enzyme kinetics or inhibitor screening studies.
Research Products International Corp Quick-DNA/RNA MiniPrep Plus Kit, 50 Preps
The Quick-DNA/RNA MiniPrep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a unique preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from any biological sample including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality genomic DNA and total RNA that is ready for any downstream application including reverse transcription, microarray, sequencing, etc.
Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Liver, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation ZR Plasmid Miniprep™-Classic Kit (400 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin IIN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
New England Biolabs, Inc. Monarch® Spin PCR & DNA Cleanup Kit - 50 preps
The Monarch Spin PCR & DNA Cleanup Kit (5 ug), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 ug of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 ul, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps
Epigentek Inc Epigenase Universal SIRT Activity/Inhibition Assay Kit (Colorimetric) (96 assays)
The Epigenase Universal SIRT Activity/Inhibition Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents for measuring the activity/inhibition of total SIRT enzymes using nuclear extracts or purified SIRT isoforms (SIRTs 1-7) from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. Detection of inhibition or activation of SIRTs is important in elucidating mechanisms of epigenetic regulation of gene activation and silencing and may benefit diagnostics and therapeutics of cancer or neurological diseases.
