RNA Preparation and Purification
Enzo Life Sciences AMPINEXT™ RNA Bisulfite-Seq Kit (Illumina) (12Reactions)
The AMPINEXT™ RNA Bisulfite-Seq Kit (Illumina) is a complete set of optimized reagents designed to carry out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit. Intended applications include whole transcriptome RNA bisulfite sequencing and various other RNA bisulfite-based next generation sequencing techniques for RNA methylation analysis. The optimized protocol and components of the kit allow the RNA to be bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using low-nanogram quantities of bisulfite converted RNA. Assay Time: 6 hours.
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 1 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
Norgen Biotek Corp Urne Cellfree Circ Rna Minikit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating RNA, including exosomal RNA as well as viral RNA from fresh, preserved or frozen urine samples from volumes ranging from 250 µL to 2 mL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Roche Diagnostics KAPA RNA HyperPrep Kit with RiboErase (HMR), 24 libraries
KAPA RNA HyperPrep Kit with RiboErase (HMR), 24 libraries. *This item is non returnable.
Roche Diagnostics KAPA RNA HyperPrep Kit, 24 libraries
KAPA RNA HyperPrep Kits include all reagents required for RNA fragmentation, cDNA synthesis, library preparation and amplification. KAPA Pure Beads are supplied in each kit for reaction cleanups, while KAPA Adapters are available separately. *This item is non returnable.
Bioworld RNase-Off Refill, 1 L
RNase Off Refill, 500MLUnique formulation destroys RNase & DNase on contact Completely washed away when rinsed Spray container prevents waste of reagents and uniformly covers area Does not contain any toxic agents or leave a residue
Bioworld RNase AWAY™ 250ml
RNase Away™ removes RNase and DNA contamination from glassware and plastic quickly and economically. Simply apply RNase away to surface or soak as needed and rinse with water. Non-abrasive formulation does not contain strong acids. Effective for use with electrophoresis equipment, glass plates, and other laboratory equipment. Specification: Removes RNase and DNA - Ready to use - Non-abrasive. Molecular Weight : 18.02 g/mol, pH : 6.0 - 8.0 at 25 °C, Solubility : completely miscible, Appearance Form : Liquid, Appearance Color : Clear, Melting Point : 0.0 °C, Boiling Point : 100.0 °C, Density : 1.023 g/cm3. 250ml
Zyagen Labs Rat thymus total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy Sprague Dawley RAT THYMUS tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Rat pituitary total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy Sprague Dawley RAT PITUITARY tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Human heart total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy HUMAN HEART tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Rat kidney total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy Sprague Dawley RAT KIDNEY tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zymo Research Corporation Quick-RNA™ MiniPrep Kit (200 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The procedure of Quick-RNA™ Miniprep Kit uses unique spin-column technology that results in highquality total RNA (including small/microRNAs) and is ready for Next-Gen Sequencing, RT/qPCR, hybridization, etcThe Quick-RNA™ Kits yields high quality total RNA.High levels of genomic DNA contamination arepresent in the preps from Suppliers Q & P but not withthe Quick-RNA™ Kits. Total RNA was isolated fromhuman epithelial cells.RNA isolated with the Quick-RNA™ Kits is DNA-free.Samples isolated with Supplier Q's kit are provided forcomparison. Total RNA was isolated from 106 humanepithelial cells (with in-column DNase treatments forboth kits). Each amplification curve represents anaverage of three independent isolation experiments.