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Sequenced positive and negative RNA controls and RNA control mixes collected from various organisms. Products include mRNA, sgRNA, tracrRNA, and crRNA and are ideal for use in gene expression experiments.
EZ Cap Cy5 EGFP mRNA (5-moUTP) expresses enhanced green fluorescent protein (EGFP) which emits green fluorescence observable at 509 nm It is suitable for assays such as mRNA delivery translation efficiency cell viability and in vivo imaging This product is provided at 1 mg/ml It is co-transcriptionally capped with a Cap 1 structure incorporated 5-moUTP (5-Methoxy-UTP) and a poly(A) tail Cy5 is a synthetic red fluorescent dye with maximum excitation and emission wavelengths at approximately 650 nm and 670 nm respectively During transcription Cy5-UTP and 5-moUTP are incorporated at a ratio of 1 3 allowing for easy visualization of mRNA while maintaining translation capability in cell culture Generally translation efficiency inversely correlates with Cy5-UTP substitution level This product is designed to mimic fully processed and mature mRNA It is an ideal product for investigating mRNA delivery localization translation and other behaviors
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The luciferase-luciferin bioluminescent system provides a highly sensitive and efficient tool for detecting gene expression In the presence of ATP oxygen and magnesium ions firefly luciferase catalyzes the oxidation reaction of luciferin into oxyluciferin accompanied by the emission of bioluminescence at about 560 nm This bioluminescence signal can be detected using a luminometer or liquid scintillation counter The Single Firefly Luciferase Reporter Gene Assay Kit allows direct quantification of intracellular firefly luciferase activity via chemiluminescence without the need for washing or harvesting cells This assay is a one-step detection kit providing exceptional sensitivity high signal stability and ease of use The luciferase detection reagent included in this kit can be directly added to cultured cells at equal volumes to the medium This assay is also suitable for measurements of previously lysed or stored cell samples
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More