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Small interfering RNA/silencing RNA (siRNA) is a class of double-stranded, non-coding RNA molecule, 20-25 base pairs in length. As part of the RNA interference pathway, it regulates gene expression by degrading mRNA after transcription, preventing translation.
A fluorescence-labelled tracer and the N-terminal tagged full-length human eIF4E/eIF4G complex are used in this assay kit. A Terbium-labeled antibody binding to the tag on eIF4E serves as a fluorescence donor (HTRF donor). The binding of the fluorescence-labeled tracer to the eIF4E brings Terbium on the anti-Tag antibody close to the fluorophore on the tracer (HTRF acceptor). Activation of the Terbium results in fluorescence resonance energy transfer (FRET). Thus, the binding status can be quantitively measured by calculating the ratio of the emission fluorescence intensity of the acceptor (665 nm) and donor (620 nm). The competitive binding of a non-fluorescence compound will reduce the FRET signal.
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Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit The protein encoded by this gene represents the catalytic subunit which uses ATP to phosphorylate PtdIns PtdIns4P and PtdIns(4 5)P2 This gene has been found to be oncogenic and has been implicated in cervical cancers A pseudogene of this gene has been defined on chromosome 22
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