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Complete and incomplete formulations of Freund's and alum-based adjuvants to mix and emulsify with processed immunogens; for use in the preparation and subcutaneous or intravenous injection of immunization agents into animals for efficient antibody production.
FA (Incomplete Freunds adjuvant) is one of the most commonly used adjuvants in research. It is prepared from non-metabolizable oils.IFA can be used to produce water-in-oil emulsions of antigens.IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells. INVIVOGEN mfg# VAC-IFA-60
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IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research. It is prepared from non-metabolizable oils. IFA can be used to produce water-in-oil emulsions of antigens. IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells.
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Synonym: FCA. Cell suspension. Each mL contains 1 mg of Mycobacterium tuberculosis (H37Ra, ATCC 25177), heat killed and dried, 0.85 mL paraffin oil and 0.15 mL mannide monooleate. Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion. Freund′s Adjuvants may be used to produce water-in-oil emulsions of immunogens. Antigens in water-in-oil emulsions stimulate high and long-lasting antibody responses which can be attributed to the slow release of antigen.
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The imidazoquinoline compound Imiquimod is a guanosine derivative and agonist for TLR7. This TLR7 agonist, originally developed as type I IFN inducers, is an effective adjuvant by activating dendritic cells (DCs) and B cells to induce cytokines optimal for Th1 cell immunity, and antibody production.
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The TLR4 agonist, MPLA (monophosphoryl lipid A) is a derivative of lipid A from Salmonella minnesota R595 lipopolysaccharide (LPS or endotoxin). MPLA is considerably less toxic than LPS whilst maintaining immunostimulatory activity.
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