Protein Labeling Reagents
Apexbio Technology LLC Cy3 maleimide (non-sulfonated), 5mg. Cas: MFCD: NA. Labeling of cysteine residues, as well as other thiolated molecules
Cy3 maleimide is a selective and efficient fluorophore dye with low aqueous solubility. This labeling reagent can be used to attach Cy3 fluorophore to proteins and peptides which contain cysteine residues, as well as to other thiolate molecules (such as thiol-containing oligonucleotides). Before the labeling reactions, Cystines should be reduced with TCEP (tris-carboxyethylphosphine). For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Other sizes are available. Please inqury us for quote.
Enzo Life Sciences BIO-PROBE 3'-Oligonucleotide labeling kit with fluorescein-12-ddUTP (25Reactions)
Terminal labeling is an ideal procedure for the labeling of oligonucleotides with haptens such as biotin or fluorescein. An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction.The BIO-PROBE 3'-Oligonucleotide Labeling Kit with Fluorescein-12-ddUTP contains all reagents required for terminal labeling of oligonucletides with fluorescein except the oligonucleotides to be labeled. The BIO-PROBE 3'-Oligonucleotide Labeling Kit provides maximum flexibility for labeling oligonucleotides approximately 15 to 100 nucleotides in length. A standard reaction will label up to 100 picomoles (1 µg of a 30-mer oligonucleotide sequence). A complete kit contains two component packs that are available separately: a Reagent Pack and a Fluorescein-12-ddUTP Dideoxynucleotide Pack. Both packs contain sufficient reagents for 25 labeling reactions. Applications: Southern Blot, Northern Blot, ISH , Dot Blot.
Biotium CellBrite™ NIR750 Cytoplasmic Membrane Dye, 2 mM in DMSO
CellBrite™ NIR Cytoplasmic Membrane Dyes are novel near-infrared carbocyanine dyes for labeling the cytoplasmic membranes of living cells. Due to their long emission wavelengths, near-infrared cell membrane stains can be used to label cells for near-infrared small animal imaging studies for non-invasive imaging of cell migration and cell homing. The dyes have long 18-carbon hydrophobic tails and an additional water-soluble group. These unique chemical structure elements make the dyes easy to dissolve while providing highly stable cytoplasmic membrane staining, unlike traditional carbocyanine dyes like DiI, DiO, and DiR, which are often difficult to dissolve or prone to precipitation during cell staining. CellBrite™ NIR750 has an excitation/emission of 748/780 nm.
Biotium Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF488A Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP goat anti-rabbit CF488A dye tyramide. The dye has an excitation/emission of 490/515 nm. The kit contains sufficient reagent for 50-150 slides.
Biotium LysoView™ 633, 100uL
LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step. Far-red fluorescent LysoView™ 633 exhibits pH-sensitive fluorescence (Ex/Em 634/659 nm at pH ≤5), further enhancing the specificity of staining.
Biotium MemBrite™ Fix 488/515 Cell Surface Staining Kit, 100 Reactions
MemBrite™ Fix Cell Surface Staining Kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments. MemBrite™ Fix are new covalent cell surface stains that react irreversibly with cell surface proteins for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. MemBrite™ Fix staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than traditional lipophilic membrane dyes like DiO, DiI, PKH, Vybrant, or CellMask™. This trial size kit has reagents sufficient for 100 labeling reactions. The kit contains 1 vial of MemBrite™ Fix 488/515 that is sufficient for 20 uL of 1000X dye after DMSO reconstitution.
Biotium LysoView™ 488, 1000X in DMSO, Trial Size
LysoView™ 405 is a blue fluorescent lysosome stain for live cells. It has excitation at 318 and 400 nm and emission at 464 nm, for detection in the DAPI or Pacific Blue channels.
Biotium Tyramide Amplification Kit with HRP Streptavidin and CF640R Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP streptavidin CF640R dye tyramide. The dye has an excitation/emission of 642/662 nm. The kit contains sufficient reagent for 50-150 slides.
Biotium LysoView™ 540, 1000X in DMSO, Trial Size
LysoView™ 540 is a red fluorescent dye that is highly selective for labeling and tracking of lysosomes in live cells, with no wash step required. With absorption and emission at 540/634 nm, the dye can be detected using a Cy3 filter set.
Biotium MemBrite™ Fix 488/515 Cell Surface Staining Kit, 500 Reactions
MemBrite™ Fix Cell Surface Staining Kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments. MemBrite™ Fix are new covalent cell surface stains that react irreversibly with cell surface proteins for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. MemBrite™ Fix staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than traditional lipophilic membrane dyes like DiO, DiI, PKH, Vybrant, or CellMask™. This kit has reagents sufficient for 500 labeling reactions. The kit contains 5 vials of MemBrite™ Fix 488/515. Each vial is sufficient for 20 uL of 1000X dye after DMSO reconstitution.
Biotium Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF640R Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP goat anti-mouse CF640R dye tyramide. The dye has an excitation/emission of 642/662 nm. The kit contains sufficient reagent for 50-150 slides.
Biotium Mix-n-Stain™ CF647 Antibody Labeling Kit, 1x(20-50ug) labeling
Mix-n-Stain™ Antibody Labeling Kits are a revolutionary antibody labeling technology that allows you to label your antibody with one of our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Choose from kit sizes to label 5-20 ug, 20-50 ug, or 50-100 ug of your antibody. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF647 dye has excitation/emission at 650/665 nm.
Biotium CF488A Maleimide
CF Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins on free thiol groups. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.