Protein Labeling Reagents
Biotium CF640R Biocytin
Fluorescent biocytin conjugates are cell-impermeant, fixable polar tracers with biotin and an aldehyde-fixable primary amine groups. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF640R dye has excitation/emission at 642/662 nm.
Biotium CF640R Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF640R dye has excitation/emission at 642/662 nm.
Biotium CF405L Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF405L dye has excitation/emission at 395/545 nm.Also see Biotium's CF Dye Tyramide Amplification Kits.
Biotium CF750, Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared fluorescent CF750 dye has excitation/emission at 755/777 nm.
Biotium Mix-n-Stain™ CF660C Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF660C dye has excitation/emission at 667/685 nm. It is suitable for labeling ligands for cell surface staining.
Biotium 5(6)-Tetramethylrhodamine Maleimide
5(6)-Carboxytetramethylrhodamine maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement for 5(6)-carboxytetramethylrhodamine C5-maleimide (91040).Properties: Ex/Em (MeOH) = 540/565 nm; Red solid soluble in DMF or DMSO; Store at -20°C and protect from light, especially in solution; C35H36N4O6; MW: 608.66.
Biotium CF770-Dextran 150, 000 MW, Anionic and Fixable
CF dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran conjugates are available in a variety of molecular weights, and contain a formaldehyde-fixable free-amine group. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red/near-IR fluorescent CF770 dye has excitation/emission at 681/698 nm.
Biotium CF514 Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF514 dye has excitation/emission at 516/548 nm.
Biotium Cyanine 555 Tyramide
Cyanine 555 tyramide (Ex/Em 555/565 nm) can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. Cyanine 555 is structurally identical to Cy3 from GE Healthcare.
STEMCELL Technologies GloCell™ Fixable Viability Dye Red 710, Size: 500 Tests
GloCell™ Fixable Viability Dye Red 710 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be used prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Red 710 is excited by the red laser (633 nm) with an excitation maximum of 647 nm, and it has a fluorescence emission maximum of 710 nm that can be detected using the 710/50 band pass filter used to detect Alexa Fluor 700.
STEMCELL Technologies GloCell™ Fixable Viability Dye Violet 450, Size: 100 Tests
GloCell™ Fixable Viability Dye Violet 450 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Violet 450 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 440/40 or 440/50 band pass filter used to detect Pacific Blue™.
Sigma Aldrich Fine Chemicals Biosciences Cardiogreen | 3599-32-4 | MFCD00013078 | 25mg
Cardiogreen | Mol Wt: 774.96 | 3599-32-4 | MFCD00013078 | 25mg
Biotium CF568 Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 dye has excitation/emission at 562/583 nm.
Sigma Aldrich Fine Chemicals Biosciences Fluorescein isothiocyanate isomer I suitable for protein labeling, >=90% (HPLC), powder | 3326-32-7 | MFCD00005063 | 5G
Fluorescein isothiocyanate isomer I suitable for protein labeling, >=90% (HPLC), powder | Purity: >=90% (HPLC) | Mol Wt: 389.38 | 3326-32-7 | MFCD00005063 | 5G